Faculty of Engineering, Department of Chemical Engineering, Mersin University , Mersin , Turkey.
Artif Cells Nanomed Biotechnol. 2014 Feb;42(1):70-7. doi: 10.3109/21691401.2013.775578. Epub 2013 Mar 11.
HEMA-Lactate-Dextran cryogel scaffolds were produced by cryogelation. Mesencyhmal stem cells (MSC) were isolated from rat bone marrow. Critical sized cranial bone defects were created in rat cranium. Stem cells were injected inside the macropores of the cryogel scaffolds prepared from HEMA-Lactate-Dextran possessing the same dimensions with the defect and placed in the cranial bone. The cryogels placed in the defect without stem cells served as control. After selected time intervals the experimental sites were removed from the animals and new bone formation and tissue integration were investigated by histological analysis. The in vivo results exhibited osseous tissue integration within the implant and mineralized functionally stable bone restoration of the cranial defects. Tissue formation started in the macrospores of the scaffold starting from periphery to the center. A significant ingrowth of connective tissue cells and new blood vessels allowed new bone formation. Histological data demonstrated that new bone per total defect area ratio, were not significantly different in "scaffold-stem cells" group compared to that of "scaffold only" group on all time points. However, the blood vessel density was significantly higher in "scaffold-stem cells" group comparing to that of the "scaffold only" group on day 30. "Scaffold-stem cells" given group gave better tissue response score when compared to "scaffold only" group on day 180.
HEMA-乳酸盐-葡聚糖冷冻凝胶支架通过冷冻凝胶法制备。间充质干细胞（MSC）从大鼠骨髓中分离出来。在大鼠颅骨中创建临界尺寸的颅骨缺损。将干细胞注射到与缺损具有相同尺寸的 HEMA-乳酸盐-葡聚糖制备的大孔冷冻凝胶支架内，并将其放置在颅骨内。将没有干细胞的冷冻凝胶放置在缺损处以作为对照。在选定的时间间隔后，将实验部位从动物体内取出，并通过组织学分析研究新骨形成和组织整合情况。体内结果显示在植入物内有骨组织整合，并且颅骨缺损得到了矿化功能稳定的骨修复。组织形成始于支架的大孔内，从外周向中心开始。结缔组织细胞和新血管的大量生长允许新骨形成。组织学数据表明，在所有时间点，“支架-干细胞”组的新骨与总缺陷面积的比例与“仅支架”组相比没有显著差异。然而，在第 30 天，“支架-干细胞”组的血管密度明显高于“仅支架”组。与“仅支架”组相比，“支架-干细胞”组在第 180 天的组织反应评分更好。
