Berski Silke, van Bergeijk Jeroen, Schwarzer David, Stark Yvonne, Kasper Cornelia, Scheper Thomas, Grothe Claudia, Gerardy-Schahn Rita, Kirschning Andreas, Dräger Gerald
Institut für Organische Chemie and Zentrum für Biomolekulare Wirkstoffe (BMWZ), Gottfried Willhelm Leibniz Universität Hannover, Schneiderberg 1B, 30167 Hannover, Germany.
Biomacromolecules. 2008 Sep;9(9):2353-9. doi: 10.1021/bm800327s. Epub 2008 Aug 9.
Restorative medicine has a constant need for improved scaffold materials. Degradable biopolymers often suffer from uncontrolled chemical or enzymatic hydrolysis by the host. The need for a second surgery on the other hand is a major drawback for nondegradable scaffold materials. In this paper we report the design and synthesis of a novel polysialic acid-based hydrogel with promising properties. Hydrogel synthesis was optimized and enzymatic degradation was studied using a phage-born endosialidase. After addition of endosialidase, hydrogels readily degraded depending on the amount of initially used cross-linker within 2 to 11 days. This polysialic acid hydrogel is not cytotoxic, completely stable under physiological conditions, and could be evaluated as growth support for PC12 cells. Here, additional coating with collagen I, poly-L-lysine or matrigel is mandatory to improve the properties of the material.
再生医学一直需要性能更优的支架材料。可降解生物聚合物常常会遭受宿主体内不受控制的化学或酶促水解。另一方面，对于不可降解的支架材料而言，需要进行二次手术是一个主要缺点。在本文中，我们报告了一种具有良好性能的新型聚唾液酸基水凝胶的设计与合成。优化了水凝胶的合成，并使用噬菌体来源的唾液酸酶研究了酶促降解。加入唾液酸酶后，水凝胶会根据最初使用的交联剂的量在2至11天内迅速降解。这种聚唾液酸水凝胶无细胞毒性，在生理条件下完全稳定，并且可以作为PC12细胞的生长支持物进行评估。在此，必须额外用I型胶原蛋白、聚-L-赖氨酸或基质胶进行包被以改善材料的性能。
