Krpata Doris, Peintner Ursula, Langer Ingrid, Fitz Walter J, Schweiger Peter
Institute of Microbiology, University Innsbruck, Technikerstrasse 25, 6020 Innsbruck, Austria.
Mycol Res. 2008 Sep;112(Pt 9):1069-79. doi: 10.1016/j.mycres.2008.02.004. Epub 2008 Mar 19.
European aspen is one of the most widely distributed trees in Central Europe and is a typical early colonizer of poor and disturbed soils. However, little is known about ectomycorrhizal (ECM) fungi in these ecosystems. We examined the ECM community of European aspen growing on a heavily contaminated site in southern Austria by analysing ECM roots, sorting them into morphotypes, subjecting them to DNA extraction, PCR, and DNA sequencing. ECM root symbionts were sampled two times in 2004. During this time, the below-ground community structure was relatively stable; we found no evidence of taxa adapted to summer or autumn conditions and only two species varied widely in occurrence between soil horizons. The ECM fungal community was diverse (54 species), rich in Basidiomycota (43 species), and dominated by Cenococcum geophilum and fungi with corticoid basidiomes (e.g. Thelephoraceae).
欧洲山杨是中欧分布最广的树木之一，是贫瘠和受干扰土壤的典型早期定居者。然而，对于这些生态系统中的外生菌根（ECM）真菌，人们了解甚少。我们通过分析ECM根，将其分类为形态型，进行DNA提取、PCR和DNA测序，研究了生长在奥地利南部一个重度污染场地的欧洲山杨的ECM群落。2004年对ECM根共生体进行了两次采样。在此期间，地下群落结构相对稳定；我们没有发现适应夏季或秋季条件的分类群的证据，并且只有两个物种在不同土壤层中的出现频率差异很大。ECM真菌群落多样（54种），担子菌门丰富（43种），以土生空团菌和具有皮质担子果的真菌（如革菌科）为主。
