Van Eyck Anne-Sophie, Jordan Bénédicte Francine, Gallez Bernard, Heilier Jean-François, Van Langendonckt Anne, Donnez Jacques
Department of Gynecology, Faculty of Medicine, Université catholique de Louvain, Louvain, Cliniques Universitaires St. Luc, Avenue Hippocrate 10, Brussels, Belgium.
Fertil Steril. 2009 Jul;92(1):374-81. doi: 10.1016/j.fertnstert.2008.05.012. Epub 2008 Aug 9.
To develop electron paramagnetic resonance (EPR) oximetry as a tool to characterize the oxygen environment in human ovarian xenografts in the early postgrafting period.
Prospective experimental study.
Gynecology research unit in a university hospital.
PATIENT(S): Biopsies were obtained from 6 women aged 22-35 years.
INTERVENTION(S): Frozen-thawed human ovarian tissue fragments were grafted to an intraperitoneal site in nude mice. Before grafting, lithium phthalocyanine, an oxygen reporter, was implanted inside the fragments.
MAIN OUTCOME MEASURE(S): To monitor partial pressure of oxygen (pO(2)) by EPR on postgrafting days 3, 5, 7, 10, 14, 17, and 21 and validate the technique by histologic assessment.
RESULT(S): A period of hypoxia was identified before day 5, followed by gradual but significant oxygenation over the next 5 days, suggesting an active process of graft revascularization. Reoxygenation kinetics in human ovarian xenotransplants were quantified.
CONCLUSION(S): Our data validated the EPR oximetry technique as a tool to monitor pO(2) in ovarian grafting. The critical early period of hypoxia was identified, and the first steps of reoxygenation were characterized. In the future, our model may be used to evaluate new freezing and grafting protocols with the aim of reducing potential cryoinjury and initial ischemia-reperfusion damage.
开发电子顺磁共振（EPR）血氧测定法，作为一种在移植后早期表征人卵巢异种移植中氧环境的工具。
前瞻性实验研究。
大学医院的妇科研究单位。
从6名年龄在22 - 35岁的女性身上获取活检组织。
将冻融后的人卵巢组织碎片移植到裸鼠的腹腔部位。在移植前，将氧报告物酞菁锂植入组织碎片内部。
在移植后第3、5、7、10、14、17和21天通过EPR监测氧分压（pO₂），并通过组织学评估验证该技术。
在第5天之前发现一段缺氧期，随后在接下来的5天内逐渐但显著地出现氧合，提示移植组织血管重建的活跃过程。对人卵巢异种移植中的再氧合动力学进行了量化。
我们的数据验证了EPR血氧测定技术作为监测卵巢移植中pO₂的工具。确定了关键的早期缺氧期，并表征了再氧合的初始步骤。未来，我们的模型可用于评估新的冷冻和移植方案，以减少潜在的冷冻损伤和初始缺血再灌注损伤。
