Baluchnejadmojarad Tourandokht, Roghani Mehrdad, Nadoushan Mohammad Reza Jalali, Bagheri Maryam
Department of Physiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Phytother Res. 2009 Jan;23(1):132-5. doi: 10.1002/ptr.2564.
A large body of experimental evidence supports a role for oxidative stress as a mediator of nerve cell death in Parkinson's disease (PD). Phytoestrogens such as genistein have been reported to prevent neuronal degeneration caused by increased oxidative burden, therefore, this study examined whether genistein administration at a high dose would attenuate behavioral and structural abnormalities in an experimental model of PD in rat. For this purpose, unilateral intrastriatal 6-hydroxydopamine (6-OHDA, 12.5 microg/5 microL of saline-ascorbate)-lesioned rats were intraperitoneally pretreated with a single and high dose of genistein (10 mg/kg) 1 h before surgery. Apomorphine-induced rotations and the number of Nissl-stained neurons in the substantia nigra pars compacta (SNC) were counted after 2 weeks. Genistein administration could attenuate the rotational behavior in lesioned rats and protect the neurons of SNC against 6-OHDA toxicity. Genistein administration has a protective effect against 6-OHDA toxicity.
大量实验证据支持氧化应激在帕金森病（PD）神经细胞死亡中起介导作用。据报道，诸如染料木黄酮等植物雌激素可预防因氧化负担增加所致的神经元变性，因此，本研究检测了高剂量给予染料木黄酮是否会减轻大鼠PD实验模型中的行为和结构异常。为此，在手术前1小时，对单侧纹状体内注射6-羟基多巴胺（6-OHDA，12.5微克/5微升生理盐水-抗坏血酸）损伤的大鼠腹腔内预先单次给予高剂量染料木黄酮（10毫克/千克）。2周后，统计阿扑吗啡诱导的旋转次数以及黑质致密部（SNC）中尼氏染色神经元的数量。给予染料木黄酮可减轻损伤大鼠的旋转行为，并保护SNC神经元免受6-OHDA毒性影响。给予染料木黄酮对6-OHDA毒性具有保护作用。
