Dan Liang, Shi-long Yan, Miao-li Lin, Yong-ping Li, Hong-jie Ma, Ying Zhang, Xiang-gui Wang
State Key Laboratory of Ophthalmology, ZhongShan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, People's Republic of China.
Curr Eye Res. 2008 Aug;33(8):653-60. doi: 10.1080/02713680802245772.
To examine the efficacy of oral doxycycline treatment (as compared to oral and topical dexamethasone) for inhibiting corneal neovascularization (CNV).
Following corneal alkali burn, rats were treated daily with oral doxycycline, oral dexamethasone, or topical dexamethasone for 14 days. Control rats were injured but were not treated. At days 3, 7, and 14 post injury, length and area of CNV were evaluated, as well as corneal epithelial healing and ulceration. Tissues were obtained from a subset of rats from each group for histopathological analysis.
At days 7 and 14 post-injury, CNV length in the doxycycline group was significantly less than in the untreated control (p < 0.008). The area of CNV was significantly smaller in doxycycline as compared to control rats on days 3, 7, and 14 post-injury. Inhibition of CNV (indicated by area and length) was significantly greater in both dexamethasone groups compared to the doxycycline group (p < 0.008 for all comparisons). However, epithelial healing was significantly more rapid in the doxycycline group compared to both dexamethasone groups (p < 0.008). Epithelial ulceration was apparent in both oral and topically treated dexamethasone rats, but not in doxycycline-treated rats.
Oral doxycycline inhibits CNV without the harmful side effects associated with dexamethasone use. Further investigation is warranted to assess the mechanisms through which doxycycline acts to cause CNV inhibition, and the applicability of doxycycline use for treating CNV in the clinical setting.
研究口服多西环素治疗（与口服及局部使用地塞米松相比）对抑制角膜新生血管化（CNV）的疗效。
大鼠角膜碱烧伤后，每日分别给予口服多西环素、口服地塞米松或局部使用地塞米松，持续14天。对照组大鼠仅受伤但未接受治疗。在受伤后第3、7和14天，评估CNV的长度和面积，以及角膜上皮愈合和溃疡情况。从每组的一部分大鼠获取组织进行组织病理学分析。
在受伤后第7天和14天，多西环素组的CNV长度显著短于未治疗的对照组（p < 0.008）。在受伤后第3、7和14天，多西环素组的CNV面积与对照大鼠相比显著更小。与多西环素组相比，两个地塞米松组对CNV的抑制作用（以面积和长度表示）显著更强（所有比较p < 0.008）。然而，与两个地塞米松组相比，多西环素组的上皮愈合明显更快（p < 0.008）。口服和局部使用地塞米松治疗的大鼠均出现上皮溃疡，而多西环素治疗的大鼠未出现。
口服多西环素可抑制CNV，且无使用地塞米松相关的有害副作用。有必要进一步研究以评估多西环素抑制CNV的作用机制，以及多西环素在临床环境中用于治疗CNV的适用性。
