与正常孕妇相比，先兆子痫患者单核细胞、自然杀伤细胞和淋巴细胞产生的细胞因子有所不同。
Cytokine production by monocytes, NK cells, and lymphocytes is different in preeclamptic patients as compared with normal pregnant women.
作者信息
van Nieuwenhoven Angelique L Veenstra, Moes Henk, Heineman Maas Jan, Santema Job, Faas Marijke M
机构信息
Department of Obstetrics and Gynecology, University Medical Centre Groningen, Groningen, The Netherlands.
出版信息
Hypertens Pregnancy. 2008;27(3):207-24. doi: 10.1080/10641950701885006.
OBJECTIVE
To measure cytokine production in ex vivo stimulated leukocyte populations of women with normal pregnancy and those with preeclampsia.
METHODS
Whole blood from preeclamptic and normal pregnant women was stimulated with LPS or PMA/Ca-ionophore. The percentages of IFN gamma and IL-2, 4, and 10 producing lymphocytes and NK cells and the percentages of TNFalpha, IL-1 beta, and IL-12 producing monocytes were measured by flowcytometry.
RESULTS
In women with preeclampsia, there was a significantly increased percentage IL-4 producing cytotoxic T cells. Also, a significant decreased percentage IL-2 producing T helper cells and IL-12 producing monocytes was seen as compared with normal pregnancy.
CONCLUSION
Th1 cytokine production of lymphocytes and monocytes appears to be decreased in our group of preeclamptic patients compared with normal pregnant women.
目的
检测正常妊娠女性和先兆子痫女性体外刺激白细胞群体中细胞因子的产生情况。
方法
用脂多糖（LPS）或佛波酯（PMA）/钙离子载体刺激先兆子痫孕妇和正常孕妇的全血。通过流式细胞术检测产生干扰素γ（IFNγ）、白细胞介素-2（IL-2）、白细胞介素-4（IL-4）和白细胞介素-10（IL-10）的淋巴细胞和自然杀伤细胞（NK细胞）的百分比，以及产生肿瘤坏死因子α（TNFα）、白细胞介素-1β（IL-1β）和白细胞介素-12（IL-12）的单核细胞的百分比。
结果
在先兆子痫女性中，产生IL-4的细胞毒性T细胞百分比显著增加。此外，与正常妊娠相比，产生IL-2的辅助性T细胞和产生IL-12的单核细胞百分比显著降低。
结论
与正常孕妇相比，我们研究的先兆子痫患者组中淋巴细胞和单核细胞的Th1细胞因子产生似乎减少。
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