Saika Shizuko, Matsunaga Teiichi, Ogawa Tomoko, Ichinohe Sadato
Chiba Prefectural Institute of Public Health.
Kansenshogaku Zasshi. 2008 Jul;82(4):310-6. doi: 10.11150/kansenshogakuzasshi1970.82.310.
Changes in gelatin particle agglutination (PA), hemagglutination inhibition (HI), and neutralization (NT) antibodies were compared using sera from 124 individuals collected between 3 weeks and 10 years after measles vaccination, and the relationship between these changes and IgG avidity was studied. PA, HI, and NT antibodies peaked 4-5 months after vaccination. The rate of increase in mean antibody titer from 0-1 months after vaccination to peak levels was 1.7-fold for NT, 1.5-fold for HI, and 7.4-fold for PA antibodies. Peak mean antibody titer was 2(11.8) for PA, 2(6.7) for NT and 2(6.7) for HI antibodies. After peaking PA antibodies changed in parallel with NT and HI antibody titers, and correlated strongly with both antibodies (r = 0.801 and 0.840). In contrast, NT and HI antibodies were consistent throughout the period. IgG avidity increased for 4-5 months following vaccination, peaking at 45%, and remaining constant at 40-50% for the next 10 years. PA antibody is strongly influenced by IgG avidity, unlike NT and HI antibodies. Due to the effects of IgG avidity, PA antibodies increase more significantly than NT and HI antibodies as IgG antibodies mature following vaccination, resulting in a weak correlation between PA and NT or HI antibodies. Following the increase in IgG avidity to maturation, PA antibodies correlated strongly with NT and HI antibodies. PA assay detected IgM antibodies against measles virus more efficiently than the NT test. The PA assay thus differs from conventional, commonly used NT and HI assays. PA assay is simple and rapid, making it very useful for detecting measles antibodies provided that its unique features are taken into accounts.
使用麻疹疫苗接种后3周龄至10岁的124名个体的血清，比较明胶颗粒凝集（PA）、血凝抑制（HI）和中和（NT）抗体的变化，并研究这些变化与IgG亲和力之间的关系。PA、HI和NT抗体在接种疫苗后4-5个月达到峰值。从接种疫苗后0-1个月至峰值水平，平均抗体滴度的增加率对于NT抗体为1.7倍，HI抗体为1.5倍，PA抗体为7.4倍。PA抗体的峰值平均抗体滴度为2（11.8），NT抗体为2（6.7），HI抗体为2（6.7）。达到峰值后，PA抗体与NT和HI抗体滴度平行变化，并且与这两种抗体均强烈相关（r = 0.801和0.840）。相比之下，NT和HI抗体在整个期间保持一致。接种疫苗后IgG亲和力增加4-5个月，峰值为45%，并在接下来的10年中保持在40-50%的恒定水平。与NT和HI抗体不同，PA抗体受IgG亲和力的强烈影响。由于IgG亲和力的作用，随着接种疫苗后IgG抗体成熟，PA抗体比NT和HI抗体增加得更显著，导致PA与NT或HI抗体之间的相关性较弱。随着IgG亲和力增加至成熟，PA抗体与NT和HI抗体强烈相关。PA检测比NT检测更有效地检测出针对麻疹病毒的IgM抗体。因此，PA检测不同于传统的常用NT和HI检测。PA检测简单快速，只要考虑到其独特特征，对于检测麻疹抗体非常有用。
