Arai Yasuhiro, Nakano Takashi, Katayama Yuko, Aoki Hiroaki, Hirayama Takanori, Ooi Yukimasa, Eda Junji, Imura Shunro, Kashiwagi Etsuro, Sano Kouichi
Department of Microbiology and Infection Control, Osaka Medical College, Osaka, Japan.
Kansenshogaku Zasshi. 2008 Jul;82(4):322-7. doi: 10.11150/kansenshogakuzasshi1970.82.322.
We applied a previously reported method to clarify whether a multidrug-resistant Shigella colonizes in a south Asian country. At Kansai Airport Quarantine Station, stool samples were collected from overseas travelers who reported a history of diarrhea. Shigella strains were isolated, ranging from 53 to 106 (average, 82.4) isolates/year (2001-2005), and almost 80% of the isolates were Shigella sonnei. The most frequent country of origin was India. Strains from the country of the most frequent origin were studied by antimicrobial susceptibility testing. Resistance to tetracycline, sulfamethoxazole-trimethoprim and nalidixic acid was observed at the highest frequency: in 23 of the 25 strains isolated in 2001, 5 of the 13 strains isolated in 2002, and 16 of the 19 strains isolated in 2005. Strains showing the most prevalent multidrug-resistance pattern were analyzed by pulsed-field gel electrophoresis (PFGE). The PFGE profiles showed that 27 of the 44 strains isolated in 2001, 2002, and 2005 were identical in PFGE pattern, as determined using two restriction enzymes. We concluded that a multidrug-resistant Shigella sonnei colonizes in a south Asian country.
我们应用一种先前报道的方法来阐明耐多药志贺氏菌是否在一个南亚国家定殖。在关西机场检疫站，从有腹泻病史的海外旅行者中采集粪便样本。分离出的志贺氏菌菌株数量为每年53至106株（平均82.4株）（2001 - 2005年），并且几乎80%的分离株为宋内志贺氏菌。最常见的来源国是印度。对来自最常见来源国的菌株进行了药敏试验研究。观察到对四环素、磺胺甲恶唑 - 甲氧苄啶和萘啶酸的耐药率最高：2001年分离的25株中有23株、2002年分离的13株中有5株以及2005年分离的19株中有16株。对显示最普遍耐多药模式的菌株进行了脉冲场凝胶电泳（PFGE）分析。PFGE图谱显示，在2001年、2002年和2005年分离的44株菌株中，有27株在使用两种限制性内切酶测定时PFGE模式相同。我们得出结论，耐多药宋内志贺氏菌在一个南亚国家定殖。
