Thust R
Zentralbl Allg Pathol. 1976;120(4):274-83.
Experiences with the behaviour of long-term cultures of experimental CNS tumours of rats induced by methylnitosourea of ethylnitrosourea, resp., are reported. Differences in cell kinetic parameters result in a selection of the best adapted cell type among those ones existing in the primary tumour. In some long-term cultures of gliomas and neurinomas an increasing polymorphism is observed by which these lines gain the morphological appearance of a glioblastoma multiforme. The loss of cell-specific morphological differentiations is a further type of anaplastic changes. In neuroectodermal tumour strains these alterations are temporarily reversible by means of addition of dibutyryl cyclic AMP to the culture fluid. There is no relationship between the type of tumour and the degree of anaplastic changes in vitro. Some cell strains remain constant for years and retain their specific properties. Their application as tester strains in neurooncological studies is recommended.
本文报道了分别用甲基亚硝基脲和乙基亚硝基脲诱导大鼠实验性中枢神经系统肿瘤进行长期培养的实验结果。细胞动力学参数的差异导致在原发肿瘤中存在的细胞类型中选择出适应性最强的细胞类型。在一些胶质瘤和神经鞘瘤的长期培养中，观察到多态性增加，这些细胞系由此获得了多形性胶质母细胞瘤的形态外观。细胞特异性形态分化的丧失是间变改变的另一种类型。在神经外胚层肿瘤细胞系中，通过向培养液中添加二丁酰环磷腺苷，这些改变可暂时逆转。肿瘤类型与体外间变改变程度之间没有关系。一些细胞系多年来保持稳定并保留其特定特性。建议将它们用作神经肿瘤学研究中的测试细胞系。
