Dausend Julia, Musyanovych Anna, Dass Martin, Walther Paul, Schrezenmeier Hubert, Landfester Katharina, Mailänder Volker
Institute of Organic Chemistry III, Macromolecular Chemistry and Organic Materials, University of Ulm, Ulm, Germany.
Macromol Biosci. 2008 Dec 8;8(12):1135-43. doi: 10.1002/mabi.200800123.
The endocytotic mechanisms involved in the uptake of charged polystyrene nanoparticles into HeLa cells were investigated. Uptake experiments were done in the presence or absence of drugs known to inhibit various factors in endocytosis. Independent of the particle charge, endocytosis is highly dependent on dynamin, F-actin, and tyrosine-specific protein kinases, which suggests a dynamin-dependent and lipid raft-dependent mechanism. However, cholesterol depletion did not hinder particle uptake. Regarding positively charged particles, macropinocytosis, the microtubule network, and cyclooxygenases are also involved. The clathrin-dependent pathway plays a minor role.
研究了带电荷的聚苯乙烯纳米颗粒进入HeLa细胞所涉及的内吞机制。在内吞作用中，分别在存在或不存在已知可抑制各种因素的药物的情况下进行摄取实验。与颗粒电荷无关，内吞作用高度依赖发动蛋白、F-肌动蛋白和酪氨酸特异性蛋白激酶，这表明存在一种依赖发动蛋白和脂筏的机制。然而，胆固醇耗竭并不妨碍颗粒摄取。对于带正电荷的颗粒，巨胞饮作用、微管网络和环氧化酶也参与其中。网格蛋白依赖性途径起次要作用。
