Institute of Organic Chemistry III - Macromolecular Chemistry and Organic Materials, University of Ulm, Albert-Einstein-Allee 11, 89081 Ulm, Germany.
Macromol Biosci. 2012 Nov;12(11):1459-71. doi: 10.1002/mabi.201200166. Epub 2012 Sep 13.
The influence of surface roughness and charge on the cellular uptake of nanoparticles in HeLa cells is investigated with fluorescent, oppositely charged, rough, and smooth nanoparticles. Flow cytometry, cLSM, and TEM reveal that rough nanoparticles are internalized by the cells more slowly and by an unidentified uptake route as no predominant endocytosis route is blocked by a variety of inhibitory drugs, while the uptake of smooth nanoparticles is strongly dependent on dynamin, F-actin, and lipid-raft. Negatively charged nanoparticles are taken up to a higher extent than positively charged ones, independent of the surface roughness.
研究了表面粗糙度和电荷对 HeLa 细胞中纳米颗粒细胞摄取的影响，使用了荧光、带相反电荷、粗糙和光滑的纳米颗粒。流式细胞术、共聚焦显微镜和 TEM 揭示，粗糙纳米颗粒被细胞摄取的速度较慢，并且通过一种未识别的摄取途径，因为各种抑制药物并不能阻断主要的内吞途径，而光滑纳米颗粒的摄取则强烈依赖于网格蛋白、肌动蛋白和脂筏。带负电荷的纳米颗粒的摄取程度高于带正电荷的纳米颗粒，而与表面粗糙度无关。
