Williams P Stephen, Hoyos Mauricio, Kurowski Pascal, Salhi Dorra, Moore Lee R, Zborowski Maciej
Department of Biomedical Engineering, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA.
Anal Chem. 2008 Sep 15;80(18):7105-15. doi: 10.1021/ac800841q. Epub 2008 Aug 13.
Split-flow thin channel (SPLITT) fractionation is a technique for continuous separation of particles or macromolecules in a fluid stream into fractions according to the lateral migration induced by application of a field perpendicular to the direction of flow. Typical applications have involved isolation of different fractions from a polydisperse sample. Some specialized applications involve the separation of the fraction influenced by the transverse field from the fraction that is not. For example, immunomagnetically labeled biological cells may be separated from nonlabeled cells with the application of a transverse magnetic field gradient. In such cases, it may be critically important to minimize contamination of the labeled cells with nonlabeled cells while at the same time maximizing the throughput. Such contamination is known as nonspecific crossover (NSC) and refers to the real or apparent migration of nonmobile particles or cells across stream lines with the mobile material. The possible mechanisms for NSC are discussed, and experimental results interpreted in terms of shear-induced diffusion (SID) caused by viscous interactions between particles in a sheared flow. It is concluded that SID may contribute to NSC, but that further experiments and mathematical modeling are necessary to more fully explore the phenomenon.
分流细通道（SPLITT）分级分离技术是一种用于将流体流中的颗粒或大分子根据垂直于流动方向施加的场引起的横向迁移连续分离成不同级分的技术。典型应用涉及从多分散样品中分离不同级分。一些特殊应用涉及将受横向场影响的级分与不受影响的级分分离。例如，通过施加横向磁场梯度，可以将免疫磁标记的生物细胞与未标记的细胞分离。在这种情况下，将标记细胞受到未标记细胞的污染降至最低，同时使通量最大化可能至关重要。这种污染称为非特异性交叉（NSC），是指不可移动的颗粒或细胞与可移动物质一起实际或明显地跨越流线迁移。讨论了NSC的可能机制，并根据剪切流中颗粒之间粘性相互作用引起的剪切诱导扩散（SID）对实验结果进行了解释。得出的结论是，SID可能导致NSC，但需要进一步的实验和数学建模来更全面地探索这一现象。
