Queensland Alliance for Agriculture and Food Innovation, University of Queensland, St. Lucia, Queensland 4072 Australia ; Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, 5885 Hollis Street, Emeryville, CA 94608 USA ; Sage Analytics, Boulder, CO 80301 USA.
Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, 5885 Hollis Street, Emeryville, CA 94608 USA.
Biotechnol Biofuels. 2015 Jul 10;8:98. doi: 10.1186/s13068-015-0279-y. eCollection 2015.
Slow-degrading, fossil fuel-derived plastics can have deleterious effects on the environment, especially marine ecosystems. The production of bio-based, biodegradable plastics from or in plants can assist in supplanting those manufactured using fossil fuels. Polyhydroxybutyrate (PHB) is one such biodegradable polyester that has been evaluated as a possible candidate for relinquishing the use of environmentally harmful plastics.
PHB, possessing similar properties to polyesters produced from non-renewable sources, has been previously engineered in sugarcane, thereby creating a high-value co-product in addition to the high biomass yield. This manuscript illustrates the coupling of a Fourier-transform infrared microspectrometer, equipped with a focal plane array (FPA) detector, with multivariate imaging to successfully identify and localize PHB aggregates. Principal component analysis imaging facilitated the mining of the abundant quantity of spectral data acquired using the FPA for distinct PHB vibrational modes. PHB was measured in the chloroplasts of mesophyll and bundle sheath cells, acquiescent with previously evaluated plant samples.
This study demonstrates the power of IR microspectroscopy to rapidly image plant sections to provide a snapshot of the chemical composition of the cell. While PHB was localized in sugarcane, this method is readily transferable to other value-added co-products in different plants.
缓慢降解的化石燃料衍生塑料会对环境造成有害影响，尤其是对海洋生态系统。从植物或植物中生产生物基、可生物降解的塑料可以帮助替代那些使用化石燃料制造的塑料。聚羟基丁酸酯（PHB）是一种可生物降解的聚酯，已被评估为可能替代对环境有害的塑料的候选材料。
PHB 具有与不可再生来源生产的聚酯相似的性质，以前曾在甘蔗中进行过工程设计，从而除了产生高生物质产量外，还创造了一种高附加值的副产物。本文展示了傅里叶变换红外显微镜与配备焦平面阵列（FPA）检测器的多变量成像相结合，成功地识别和定位了 PHB 聚集体。主成分分析成像有助于挖掘 FPA 获得的大量光谱数据，以用于 PHB 振动模式的独特分析。在叶肉和束鞘细胞的叶绿体中测量到 PHB，与以前评估的植物样品一致。
这项研究展示了红外微光谱学快速成像植物切片以提供细胞化学成分快照的能力。虽然 PHB 已在甘蔗中定位，但这种方法很容易转移到其他不同植物中的其他高附加值副产物。
