Department of Medical Chemistry, Biochemistry and Biotechnology, Center of Excellence on Neurodegenerative Diseases, University of Milan, Via Fratelli Cervi 93, 20090 Segrate, Italy.
Glycoconj J. 2009 Nov;26(8):961-73. doi: 10.1007/s10719-008-9145-5.
Thin layer chromatography is the easiest way to analyze the total glycosphingolipid mixtures extracted, and, in some cases, partially purified from tissues and cultured cells. Several solvent systems have been introduced to separate the complex mixtures as a function of their composition, presence of contaminants and, in some cases, of their quantity. In addition, colorimetric, enzymatic, immunological and radiochemical detection procedures are available for their recognition. The method does not allow to determine the chemical structure of separated molecules, but gives a very economical and very accessible first information on their possible structure on the basis of their chromatographic mobility in comparison with standards, and of their reactivity to the staining procedures. In this paper we show how to perform mono and two-dimensional thin layer chromatography of the total lipid mixture extracted from mouse brains and, in a few cases, from cells in culture. Table 1 shows the structures of reported lipids.
薄层层析是分析从组织和培养细胞中提取的、总糖脂混合物的最简单方法，在某些情况下，也可以对其进行部分纯化。已经引入了几种溶剂系统来分离复杂的混合物，这取决于它们的组成、存在的污染物，在某些情况下，还取决于它们的数量。此外，还可以使用比色、酶、免疫和放射化学检测程序来识别它们。该方法不能确定分离分子的化学结构，但可以根据其在与标准品的比较中的色谱迁移率，以及对染色程序的反应性，提供关于其可能结构的非常经济且非常容易获得的初步信息。本文展示了如何对从小鼠大脑中提取的总脂混合物，以及在某些情况下从培养细胞中提取的总脂混合物进行单维及二维薄层层析。表 1 显示了报告的脂质的结构。
