[阿根廷分离的单核细胞增生李斯特菌血清型的多重聚合酶链反应研究]

[Study by multiplex PCR of Listeria monocytogenes serotypes isolated in Argentine].

作者信息

Callejo R, Prieto M, Martínez C, Aguerre L, Rocca F, Martínez G, Palmieri O

机构信息

Departamento Bacteriología, Instituto Nacional de Enfermedades Infecciosas, Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos G. Malbrán, Ciudad Autónoma de Buenos Aires, Argentina.

出版信息

Rev Argent Microbiol. 2008 Apr-Jun;40(2):89-92.

PMID:18705488

Abstract

A multiplex PCR assay, recently validated to characterize the serotypes of Listeria monocytogenes was evaluated in comparison to conventional serotyping. Three hundred forty two L. monocytogenes strains isolated from human, food, animal and environmental sources during the 1992-2005 period were assayed. The concordance between the two methods for serotypes 1/2a, 1/2b and 1/2c was 100%, whereas for serotype 4b it was 98%. Serotyping is a useful tool for first line strain differentiation during epidemiological surveillance and outbreaks. The multiplex PCR assay offers a fast and low-cost alternative, which is easily adaptable to clinical bacteriology and bromatology laboratories.

摘要

最近验证的一种用于鉴定单核细胞增生李斯特菌血清型的多重PCR检测方法，与传统血清分型法相比进行了评估。对1992年至2005年期间从人类、食品、动物和环境来源分离的342株单核细胞增生李斯特菌菌株进行了检测。两种方法对血清型1/2a、1/2b和1/2c的一致性为100%，而血清型4b的一致性为98%。血清分型是流行病学监测和疫情暴发期间一线菌株鉴别中的有用工具。多重PCR检测提供了一种快速且低成本的替代方法，很容易应用于临床细菌学和食品科学实验室。