Schenkein Jeremy G, Park Saeyoung, Nahm Moon H
University of Alabama at Birmingham, Pathology, Birmingham, AL 35294, United States.
Vaccine. 2008 Oct 9;26(43):5521-6. doi: 10.1016/j.vaccine.2008.07.071. Epub 2008 Aug 13.
The primary mode of prevention of adult disease from Streptococcus pneumoniae is vaccination with anti-capsular polysaccharide vaccine; however, its effects are less in the targeted older population than in young persons. Few studies have examined the mechanism behind this limited effectiveness. We have measured antibody concentrations and opsonization titers for multiple serotypes amongst both old adults and young, healthy controls. To avoid specificity problems associated with pneumococcal antibody ELISA, we absorbed the serum samples with c-polysaccharide and capsular polysaccharide of 22F type. Antibody concentrations were found to be similar for six out of the seven tested serotypes, while opsonization titers were significantly higher in six out of seven serotypes in the younger population. Antibody potency, as measured by the ratio of opsonization titer to antibody concentration, was found to be significantly higher for the younger subjects for all serotypes. We conclude that, while all ages of adults make similar concentrations of antibodies in response to pneumococcal vaccine, the effectiveness of those antibodies is significantly reduced in the older adult population.
预防成人感染肺炎链球菌疾病的主要方式是接种抗荚膜多糖疫苗；然而，该疫苗在目标老年人群中的效果不如在年轻人中显著。很少有研究探究这种有限有效性背后的机制。我们测量了老年人和年轻健康对照人群中多种血清型的抗体浓度和调理吞噬效价。为避免与肺炎球菌抗体ELISA相关的特异性问题，我们用c-多糖和22F型荚膜多糖吸附血清样本。结果发现，在七种测试血清型中，有六种血清型的抗体浓度相似，而在七种血清型中，有六种血清型在年轻人群中的调理吞噬效价显著更高。通过调理吞噬效价与抗体浓度之比衡量的抗体效力，在所有血清型中，年轻受试者的抗体效力均显著更高。我们得出结论，虽然所有年龄段的成年人对肺炎球菌疫苗产生的抗体浓度相似，但这些抗体在老年人群中的有效性显著降低。
