Mas Emilie, Michel Françoise, Guy Alexandre, Bultel Valérie, Falquet Yoan, Chardon Patrick, Rossi Jean-Claude, Cristol Jean Paul, Durand Thierry
Department of Biochemistry, Lapeyronie Hospital, Montpellier, France.
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Sep 1;872(1-2):133-40. doi: 10.1016/j.jchromb.2008.07.037. Epub 2008 Aug 3.
Isoprostanes are a family of prostaglandin isomers produced from oxidation of polyunsaturated fatty acids through a non-enzymatic free radical-catalyzed mechanism. Quantification of F(2)-isoprostanes (F(2)-IsoPs) provides a good index of oxidative stress and allows non-invasive assessment of lipid peroxidation in vivo. Since "interferences peaks" at m/z 573 co-elute with d(4)-15-F(2t)-IsoP preferentially used, we propose a new GC-NICI-MS approach to quantify urinary F(2)-IsoPs by using 4(RS)-F(4t)-neuroprostane as the internal standard. This method was applied to quantify urinary F(2)-IsoPs excretion in healthy volunteers and polytraumatized patients. Our results showed a significant increase (p<0.0001) in urinary F(2)-IsoPs in polytraumatized patients compared with healthy volunteers (4.73+/-2.75 ng/mg vs. 0.811+/-0.359 ng/mg creatinine).
异前列腺素是一族通过非酶自由基催化机制由多不饱和脂肪酸氧化产生的前列腺素异构体。F(2)-异前列腺素（F(2)-IsoPs）的定量分析提供了一个良好的氧化应激指标，并允许对体内脂质过氧化进行非侵入性评估。由于在m/z 573处的“干扰峰”与优先使用的d(4)-15-F(2t)-IsoP共洗脱，我们提出了一种新的气相色谱-负离子化学电离质谱方法，以4(RS)-F(4t)-神经前列腺素作为内标来定量尿中F(2)-IsoPs。该方法应用于定量健康志愿者和多发伤患者尿中F(2)-IsoPs的排泄量。我们的结果显示，与健康志愿者相比，多发伤患者尿中F(2)-IsoPs显著增加（p<0.0001）（4.73±2.75 ng/mg对0.811±0.359 ng/mg肌酐）。
