Milne Ginger L, Yin Huiyong, Brooks Joshua D, Sanchez Stephanie, Jackson Roberts L, Morrow Jason D
Division of Clinical Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.
Methods Enzymol. 2007;433:113-26. doi: 10.1016/S0076-6879(07)33006-1.
Oxidant stress has been implicated in a wide variety of disease processes. One method to quantify oxidative injury is to measure lipid peroxidation. Quantification of a group of prostaglandin F(2)-like compounds derived from the nonezymatic oxidation of arachidonic acid, termed the F(2)-isoprostanes (F(2)-IsoPs), provides an accurate assessment of oxidative stress both in vitro and in vivo. In fact, in a recent National Institutes of Health-sponsored independent study, F(2)-IsoPs were shown to be the most reliable index of in vivo oxidant stress when compared against other well-known biomarkers. This article summarizes current methodology used to quantify these molecules. Our laboratory's method to measure F(2)-IsoPs in biological fluids and tissues using gas chromatography-mass spectrometry is detailed herein. In addition, other mass spectrometric approaches, as well as immunological methods to measure these compounds, are discussed. Finally, the utility of these molecules as in vivo biomarkers of oxidative stress is summarized.
氧化应激与多种疾病过程相关。量化氧化损伤的一种方法是测量脂质过氧化。对一组由花生四烯酸非酶氧化产生的前列腺素F2样化合物（称为F2-异前列腺素，F2-IsoPs）进行定量，可在体外和体内准确评估氧化应激。事实上，在最近一项由美国国立卫生研究院资助的独立研究中，与其他知名生物标志物相比，F2-IsoPs被证明是体内氧化应激最可靠的指标。本文总结了目前用于量化这些分子的方法。本文详细介绍了我们实验室使用气相色谱-质谱法测量生物体液和组织中F2-IsoPs的方法。此外，还讨论了其他质谱方法以及测量这些化合物的免疫方法。最后，总结了这些分子作为氧化应激体内生物标志物的效用。
