L-二氨基丁酸肽核酸（L-dabPNA）与气单胞菌肽核酸（aegPNA）之间形成复合物的证据。

Evidences for complex formation between L-dabPNA and aegPNA.

作者信息

Roviello Giovanni N, Musumeci Domenica, Bucci Enrico M, Castiglione Mariangela, Cesarani Annalisa, Pedone Carlo, Piccialli Gennaro

机构信息

Istituto di Biostrutture e Bioimmagini-CNR, via Mezzocannone 16, I-80134 Napoli, Italy.

出版信息

Bioorg Med Chem Lett. 2008 Sep 1;18(17):4757-60. doi: 10.1016/j.bmcl.2008.08.005. Epub 2008 Aug 6.

DOI:10.1016/j.bmcl.2008.08.005

PMID:18707882

Abstract

Continuing our research on the development of nucleopeptides as ODN analogs for biomedical and bioengineering applications, here we report the synthesis and the chemical-physical characterization of a homoadenine hexamer based on a l-diaminobutyric acid (l-DABA) backbone (dabPNA), and its binding studies with a complementary aegPNA. We demonstrated by CD and UV experiments that the l-dabPNA binds the aegPNA forming a complex with good thermal stability, that we identified as a left-handed triplex.

摘要

继续我们关于开发核肽作为用于生物医学和生物工程应用的寡核苷酸类似物的研究，在此我们报告基于L-二氨基丁酸（L-DABA）主链的同型腺嘌呤六聚体（dabPNA）的合成及其化学物理特性，以及它与互补aegPNA的结合研究。我们通过圆二色光谱（CD）和紫外实验证明，L-dabPNA与aegPNA结合形成具有良好热稳定性的复合物，我们将其鉴定为左手三链体。