Kumar Ashok, Bhardwaj Aditi
Department of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur, 208016 Kanpur, India.
Biomed Mater. 2008 Sep;3(3):034008. doi: 10.1088/1748-6041/3/3/034008. Epub 2008 Aug 15.
The analysis and separation of cells including rare cell populations from a heterogeneous population has become an important tool in scientific research and medicine. The last two decades have seen tremendous progress in the field of cell separation, as a result of which various methods have been available which utilize different physiochemical and immunological characteristics of cells. A brief overview of methods of cell separation used in the past and a detailed description of methods being used presently as well as prospective methods are discussed in the paper. Chromatography has come into the picture as the apparent solution for the limitations of various cell separation methods. In recent years, a new and promising affinity-based cell separation chromatography technique has been developed using highly interconnected supermacroporous cryogels, and this new method is discussed in detail. Due to the importance and relevance of cell-based therapy and stem cell separation in the present scenario, cryogels present a new tool for preparative scale cell separation.
从异质群体中分析和分离细胞（包括稀有细胞群体）已成为科学研究和医学中的一项重要工具。在过去二十年中，细胞分离领域取得了巨大进展，因此出现了各种利用细胞不同物理化学和免疫学特性的方法。本文讨论了过去使用的细胞分离方法的简要概述、目前正在使用的方法以及未来方法的详细描述。色谱法已成为解决各种细胞分离方法局限性的明显解决方案。近年来，一种使用高度互连的超大孔冷冻凝胶开发的基于亲和力的新型细胞分离色谱技术崭露头角，本文将详细讨论这种新方法。鉴于基于细胞的疗法和干细胞分离在当前情况下的重要性和相关性，冷冻凝胶为制备规模的细胞分离提供了一种新工具。
