Guinée E P, Beuman G H, Hageman G, Welle I J, Kleinjans J C
Department of Biological Health Science, University of Limburg, Maastricht, The Netherlands.
Pharm Weekbl Sci. 1991 Apr 26;13(2):78-82. doi: 10.1007/BF01974985.
The genotoxic risk of handling antineoplastic drugs was evaluated in fifteen women preparing chemotherapeutics in the Pharmacy Department of the University Hospital Maastricht. Twenty nurses of the same hospital, who were not exposed to cytostatics, acted as controls. Endogenous exposure to antineoplastic drugs was assessed by determination of urine mutagenicity, as well as by analysis of urinary methotrexate levels. As genotoxicological end-points, sister chromatid exchanges and hypoxanthine guanine phosphoribosyl transferase locus point mutations were studied in peripheral lymphocytes obtained via venous puncture. No differences in urine mutagenic activity, in sister chromatid exchange frequencies and in hypoxanthine guanine phosphoribosyl transferase point mutation frequencies between exposed and non-exposed groups were detected. Higher sister chromatid exchange frequency was observed in smokers as compared to non-smokers.
在马斯特里赫特大学医院药房配制化疗药物的15名女性中，评估了处理抗肿瘤药物的遗传毒性风险。同一家医院的20名未接触细胞抑制剂的护士作为对照。通过测定尿液致突变性以及分析尿中甲氨蝶呤水平来评估内源性抗肿瘤药物暴露情况。作为遗传毒理学终点，在通过静脉穿刺获得的外周淋巴细胞中研究了姐妹染色单体交换和次黄嘌呤鸟嘌呤磷酸核糖转移酶基因座点突变。未检测到暴露组和未暴露组之间在尿液诱变活性、姐妹染色单体交换频率和次黄嘌呤鸟嘌呤磷酸核糖转移酶点突变频率方面存在差异。与不吸烟者相比，吸烟者的姐妹染色单体交换频率更高。
