Mahmoodi Majid, Soleyman-Jahi Saeed, Zendehdel Kazem, Mozdarani Hossein, Azimi Cyrus, Farzanfar Farideh, Safari Zahra, Mohagheghi Mohammad-Ali, Khaleghian Malihea, Divsalar Kouros, Asgari Esfandiar, Rezaei Nima
a Cancer Research Center, Cancer Institute, Tehran University of Medical Sciences , Tehran , Iran.
b Department of Medical Genetics , Faculty of Medical Sciences, Tarbiat Modares University , Tehran , Iran.
Drug Chem Toxicol. 2017 Apr;40(2):235-240. doi: 10.1080/01480545.2016.1209678. Epub 2016 Jul 27.
Concern exists regarding the possible hazards to the personnel handling anti-neoplastic drugs. The purpose of the present study was to assess the genotoxicity induced by anti-neoplastic agents in oncology department personnel.
To do this, the frequency of chromosomal aberrations (CAs) induced in peripheral blood lymphocytes was assessed at G phase of the cell cycle using metaphase analysis, cytokinesis block-micronucleus (MN) assay and sister chromatid exchange (SCE) assay. These cytogenetic end points were measured among 71 nurses in oncology department and 10 drugstore personnel handling and preparing anti-neoplastic drugs. The results were compared to those of 74 matched nurses for age and sex not exposed to any anti-neoplastic agents.
There was no significant difference between the age of study subjects and control group (p > 0.05). The results showed that the mean frequency of cytogenetic damages in terms of CAs [chromatid breaks (p = 0.01), chromosome breaks (p = 0.005), total CAs (p = 0.001)], MN formation (p = 0.001), and SCE (p = 0.004) in lymphocytes of personnel handling anti-neoplastic drugs were significantly higher than those in control unexposed group.
Results of the present study demonstrate the cytogenetic damage in peripheral blood lymphocytes of oncology department personnel. Suitable training and proper knowledge when handling anti-neoplastic drugs are emphasized to avoid potential health hazards caused by cytostatic agents.
人们担心处理抗肿瘤药物可能对工作人员造成危害。本研究的目的是评估抗肿瘤药物对肿瘤科工作人员的遗传毒性。
为此，使用中期分析、胞质分裂阻滞微核（MN）试验和姐妹染色单体交换（SCE）试验，在细胞周期的G期评估外周血淋巴细胞中诱导的染色体畸变（CA）频率。在71名肿瘤科护士和10名处理和配制抗肿瘤药物的药店工作人员中测量这些细胞遗传学终点。将结果与74名年龄和性别匹配且未接触任何抗肿瘤药物的护士的结果进行比较。
研究对象与对照组的年龄无显著差异（p＞0.05）。结果显示，处理抗肿瘤药物的人员淋巴细胞中，就CA而言，染色单体断裂（p = 0.01）、染色体断裂（p = 0.005）、总CA（p = 0.001）、MN形成（p = 0.001）和SCE（p = 0.004）的细胞遗传学损伤平均频率显著高于未接触对照组。
本研究结果表明肿瘤科工作人员外周血淋巴细胞存在细胞遗传学损伤。强调在处理抗肿瘤药物时进行适当培训和掌握相关知识，以避免细胞毒性药物造成潜在健康危害。
