PCR amplification of mRNA directly from a crude cell lysate prepared by thermophilic protease digestion.
作者信息
Fung M C, Fung K Y
机构信息
Division of Biochemistry and Molecular Biology, John Curtin School of Medical Research, Australian National University, Canberra.
出版信息
Nucleic Acids Res. 1991 Aug 11;19(15):4300. doi: 10.1093/nar/19.15.4300.
Abstract
摘要
相似文献
1
PCR amplification of mRNA directly from a crude cell lysate prepared by thermophilic protease digestion.通过嗜热蛋白酶消化制备的粗细胞裂解物直接进行mRNA的PCR扩增。
Nucleic Acids Res. 1991 Aug 11;19(15):4300. doi: 10.1093/nar/19.15.4300.
2
Salt-dependent performance variation of DNA polymerases in co-amplification PCR.共扩增PCR中DNA聚合酶的盐依赖性性能变化
Biotechniques. 1996 Jul;21(1):28-30. doi: 10.2144/96211bm04.
3
Minimizing deletion mutagenesis artifact during Taq DNA polymerase PCR by E. coli SSB.利用大肠杆菌单链结合蛋白在Taq DNA聚合酶PCR过程中减少缺失诱变假象
Nucleic Acids Res. 1992 Aug 25;20(16):4371. doi: 10.1093/nar/20.16.4371.
4
Amplification, detection, and automated sequencing of gibbon interleukin-2 mRNA by Thermus aquaticus DNA polymerase reverse transcription and polymerase chain reaction.用嗜热水生菌DNA聚合酶逆转录和聚合酶链反应对长臂猿白细胞介素-2 mRNA进行扩增、检测及自动测序
Anal Biochem. 1990 Nov 1;190(2):292-6. doi: 10.1016/0003-2697(90)90196-g.
5
Producing single-stranded DNA probes with the Taq DNA polymerase: a high yield protocol.利用Taq DNA聚合酶制备单链DNA探针：一种高产方案。
Biotechniques. 1991 Jan;10(1):35-6, 38-9.
6
Direct sequencing of double-stranded polymerase chain reaction-amplified 16S rDNA.双链聚合酶链反应扩增的16S核糖体DNA的直接测序
Anal Biochem. 1991 Dec;199(2):216-8. doi: 10.1016/0003-2697(91)90092-8.
7
[Use of thermostable DNA polymerase from Thermus thermophilus KTP in a combined reverse transcription and amplification reaction of detecting interleukin 2alpha RNA and determining expression of the multidrug resistance gene (MDR-1)].嗜热栖热菌KTP的热稳定DNA聚合酶在检测白细胞介素2α RNA和测定多药耐药基因（MDR-1）表达的逆转录与扩增联合反应中的应用
Mol Biol (Mosk). 1995 Jul-Aug;29(4):930-41.
8
Incomplete primer extension during in vitro DNA amplification catalyzed by Taq polymerase; exploitation for DNA sequencing.Taq聚合酶催化的体外DNA扩增过程中的不完全引物延伸；用于DNA测序的应用
Nucleic Acids Res. 1989 Dec 11;17(23):9613-20. doi: 10.1093/nar/17.23.9613.
9
Fidelity of Thermococcus litoralis DNA polymerase (Vent) in PCR determined by denaturing gradient gel electrophoresis.通过变性梯度凝胶电泳测定嗜热栖热菌DNA聚合酶（Vent）在聚合酶链式反应中的保真度。
Nucleic Acids Res. 1991 Aug 11;19(15):4193-8. doi: 10.1093/nar/19.15.4193.
10
Renin gene expression in various tissues determined by single-step polymerase chain reaction.通过单步聚合酶链反应测定各种组织中的肾素基因表达。
Clin Exp Pharmacol Physiol. 1991 May;18(5):357-62. doi: 10.1111/j.1440-1681.1991.tb01463.x.
引用本文的文献
1
Overexpression and characterization of thermostable serine protease in Escherichia coli encoded by the ORF TTE0824 from Thermoanaerobacter tengcongensis.嗜热栖热放线菌ORF TTE0824编码的耐热丝氨酸蛋白酶在大肠杆菌中的过表达及特性研究
Extremophiles. 2007 Nov;11(6):769-79. doi: 10.1007/s00792-007-0103-0. Epub 2007 Jul 27.
2
Detection of mRNA by reverse transcription-PCR as an indicator of viability in Escherichia coli cells.通过逆转录-聚合酶链反应检测mRNA作为大肠杆菌细胞活力的指标。
Appl Environ Microbiol. 1998 Apr;64(4):1313-8. doi: 10.1128/AEM.64.4.1313-1318.1998.
3
RT-PCR with affinity-captured mRNA.采用亲和捕获mRNA的逆转录-聚合酶链反应
Nucleic Acids Res. 1993 May 11;21(9):2281. doi: 10.1093/nar/21.9.2281.
本文引用的文献
1
Developmental expression of PDGF, TGF-alpha, and TGF-beta genes in preimplantation mouse embryos.血小板衍生生长因子、转化生长因子-α和转化生长因子-β基因在小鼠植入前胚胎中的发育表达。
Science. 1988 Sep 30;241(4874):1823-5. doi: 10.1126/science.3175624.
2
Wound macrophages express TGF-alpha and other growth factors in vivo: analysis by mRNA phenotyping.伤口巨噬细胞在体内表达转化生长因子-α及其他生长因子：通过mRNA表型分析
Science. 1988 Aug 5;241(4866):708-12. doi: 10.1126/science.3041594.
3
Preparation of crude cell extract suitable for amplification of RNA by the polymerase chain reaction.制备适用于通过聚合酶链反应扩增RNA的粗细胞提取物。
Nucleic Acids Res. 1989 Mar 11;17(5):2141. doi: 10.1093/nar/17.5.2141.
4
Message amplification phenotyping (MAPPing): a technique to simultaneously measure multiple mRNAs from small numbers of cells.信号放大表型分析（MAPPing）：一种同时测量少量细胞中多种mRNA的技术。
Biotechniques. 1989 Nov-Dec;7(10):1096-103.
5
The genes for leukemia inhibitory factor and interleukin-6 are expressed in mouse blastocysts prior to the onset of hemopoiesis.白血病抑制因子和白细胞介素-6的基因在小鼠胚泡造血开始前就已表达。
Mol Cell Biol. 1990 Sep;10(9):4953-6. doi: 10.1128/mcb.10.9.4953-4956.1990.
Zotero 插件