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PCR amplification of mRNA directly from a crude cell lysate prepared by thermophilic protease digestion.

作者信息

Fung M C, Fung K Y

机构信息

Division of Biochemistry and Molecular Biology, John Curtin School of Medical Research, Australian National University, Canberra.

出版信息

Nucleic Acids Res. 1991 Aug 11;19(15):4300. doi: 10.1093/nar/19.15.4300.

DOI:10.1093/nar/19.15.4300
PMID:1870987
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC328586/
Abstract
摘要

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本文引用的文献

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Developmental expression of PDGF, TGF-alpha, and TGF-beta genes in preimplantation mouse embryos.血小板衍生生长因子、转化生长因子-α和转化生长因子-β基因在小鼠植入前胚胎中的发育表达。
Science. 1988 Sep 30;241(4874):1823-5. doi: 10.1126/science.3175624.
2
Wound macrophages express TGF-alpha and other growth factors in vivo: analysis by mRNA phenotyping.伤口巨噬细胞在体内表达转化生长因子-α及其他生长因子：通过mRNA表型分析
Science. 1988 Aug 5;241(4866):708-12. doi: 10.1126/science.3041594.
3
Preparation of crude cell extract suitable for amplification of RNA by the polymerase chain reaction.制备适用于通过聚合酶链反应扩增RNA的粗细胞提取物。
Nucleic Acids Res. 1989 Mar 11;17(5):2141. doi: 10.1093/nar/17.5.2141.
4
Message amplification phenotyping (MAPPing): a technique to simultaneously measure multiple mRNAs from small numbers of cells.信号放大表型分析（MAPPing）：一种同时测量少量细胞中多种mRNA的技术。
Biotechniques. 1989 Nov-Dec;7(10):1096-103.
5
The genes for leukemia inhibitory factor and interleukin-6 are expressed in mouse blastocysts prior to the onset of hemopoiesis.白血病抑制因子和白细胞介素-6的基因在小鼠胚泡造血开始前就已表达。
Mol Cell Biol. 1990 Sep;10(9):4953-6. doi: 10.1128/mcb.10.9.4953-4956.1990.