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白血病抑制因子和白细胞介素-6的基因在小鼠胚泡造血开始前就已表达。

The genes for leukemia inhibitory factor and interleukin-6 are expressed in mouse blastocysts prior to the onset of hemopoiesis.

作者信息

Murray R, Lee F, Chiu C P

机构信息

Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, California 94304.

出版信息

Mol Cell Biol. 1990 Sep;10(9):4953-6. doi: 10.1128/mcb.10.9.4953-4956.1990.

DOI:10.1128/mcb.10.9.4953-4956.1990
PMID:2117704
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC361118/
Abstract

We have investigated the role that hemopoietic regulatory molecules may play in mouse embryogenesis prior to the appearance of hemopoietic stem cells or their microenvironments. Using polymerase chain reaction analysis, we detected mRNA transcripts for interleukin-6 (IL-6) and leukemia inhibitory factor (LIF) but not for granulocyte-macrophage colony-stimulating factor (GM-CSF) or IL-3 in mouse blastocysts at 3.5 days of gestation. Functional IL-6 protein was also detected in cultured blastocysts as a secreted product, as was an activity consistent with the presence of LIF protein. The expression of IL-6 and LIF in blastocysts prior to hemopoiesis suggests that these proteins may regulate the growth and development of trophoblasts or embryonic stem cells.

摘要

我们已经研究了造血调节分子在造血干细胞或其微环境出现之前在小鼠胚胎发生过程中可能发挥的作用。通过聚合酶链反应分析，我们在妊娠3.5天的小鼠囊胚中检测到白细胞介素-6（IL-6）和白血病抑制因子（LIF）的mRNA转录本，但未检测到粒细胞-巨噬细胞集落刺激因子（GM-CSF）或IL-3的转录本。在培养的囊胚中也检测到功能性IL-6蛋白作为分泌产物，同时还检测到与LIF蛋白存在一致的活性。造血发生之前囊胚中IL-6和LIF的表达表明这些蛋白质可能调节滋养层细胞或胚胎干细胞的生长和发育。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7155/361118/c5c1bb3c9e2f/molcellb00045-0526-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7155/361118/67a1b7f38114/molcellb00045-0526-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7155/361118/c5c1bb3c9e2f/molcellb00045-0526-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7155/361118/67a1b7f38114/molcellb00045-0526-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7155/361118/c5c1bb3c9e2f/molcellb00045-0526-b.jpg

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