Das S R, Ghoshal S
Ann Trop Med Parasitol. 1976 Dec;70(4):439-43. doi: 10.1080/00034983.1976.11687144.
A virulent strain of Entamoeba histolytica (NIH-200) from a symptomatic human case, cultured in vitro for more than three years, was unable to infect rat caecum or hamster liver. When grown with a mixed bacterial flora, it infected rat caecum but did not produce ulceration. After passaging amoebae growing with bacteria in hamster liver, amoebic hepatic lesions were regularly produced. The amoebae from liver lesions produced ulceration in the caeca of rats. Expoxure to cholesterol of amoebae growing with bacteria or feeding rats orally with cholesterol and injecting these amoebae along with bacteria also led to the production of ulcers in rats. Thus virulent amoebae which have been cultured in vitro for a long time become attenuated but their virulence can be revived.
从一名有症状的人类病例中分离出的一株强毒力溶组织内阿米巴（NIH - 200），在体外培养三年多后，无法感染大鼠盲肠或仓鼠肝脏。当与混合菌群一起培养时，它能感染大鼠盲肠但不产生溃疡。在仓鼠肝脏中传代培养与细菌一起生长的阿米巴后，能定期产生阿米巴性肝损伤。来自肝损伤的阿米巴在大鼠盲肠中产生溃疡。将与细菌一起生长的阿米巴暴露于胆固醇中，或给大鼠口服胆固醇并将这些阿米巴与细菌一起注射，也会导致大鼠产生溃疡。因此，长期在体外培养的强毒力阿米巴会减弱，但它们的毒力可以恢复。
