Vinayak V K, Sawhney S, Jain P, Chugh S, Naik S R, Chakravarti R N
Trans R Soc Trop Med Hyg. 1981;75(1):32-7. doi: 10.1016/0035-9203(81)90010-9.
The virulence of 70 isolates of Entamoeba histolytica was studied in the Wistar strain of albino rat by intracaecal inoculation of amoebae. The results were evaluated by the Neal scoring system, histopathological grading of ulcers and virulence indices. It was found that isolates from acute amoebic cases could infect and produce ulcers in significantly more animals than could the isolates from asymptomatic cyst passers. The virulence indices of isolates from acute cases were also significantly higher as compared to those from asymptomatic cyst passers. However, there was no significant difference between virulence indices of isolates from acute cases and non-dysenteric amoebic colitis. This study also showed that Neal scores failed to correlate with the type of pathology produced by the amoebae in the caecum. It is felt that virulence indices which depend upon the histopathological lesions should also be taken into consideration. The serological response of the patients tended to correlate with the virulence indices of isolates but only minimally. The results thus contradict a common belief amongst clinicians that higher levels of anti-amoebic antibodies reflect severe disease, as compared to low levels of antiamoebic antibodies.
通过向盲肠内接种变形虫，在白化病Wistar大鼠品系中研究了70株溶组织内阿米巴的毒力。结果采用Neal评分系统、溃疡的组织病理学分级和毒力指数进行评估。发现来自急性阿米巴病例的分离株比来自无症状排包囊者的分离株能感染更多动物并产生溃疡。与无症状排包囊者的分离株相比，急性病例分离株的毒力指数也显著更高。然而，急性病例分离株与非痢疾性阿米巴结肠炎分离株的毒力指数之间没有显著差异。本研究还表明，Neal评分与盲肠内变形虫产生的病理类型无关。认为还应考虑依赖于组织病理学病变的毒力指数。患者的血清学反应倾向于与分离株的毒力指数相关，但相关性很小。因此，结果与临床医生的普遍看法相矛盾，即与低水平抗阿米巴抗体相比，高水平抗阿米巴抗体反映严重疾病。
