Woerly Stéphane, Fort Sébastien, Pignot-Paintrand Isabelle, Cottet Cécile, Carcenac Carole, Savasta Marc
Centre de Recherches sur les Macromolecules Vegetales (CERMAV-CNRS), BP 53, 38041 Grenoble Cedex 09, France.
Biomacromolecules. 2008 Sep;9(9):2329-37. doi: 10.1021/bm800234r. Epub 2008 Aug 21.
This study describes the preparation and the characterization of poly[ N-(2-hydroxypropyl methacrylamide)] hydrogel with bulk-modified saccharidic portion of ganglioside GM 3 (Neu5Ac-alpha2,3-Gal-beta1,4-Glc). The 3'-sialyllactose is a bioactive epitope recognized by many cell surface receptors on viruses, bacteria, and human cells such as growth factor receptors. Acrylated 3'-sialyllactose was synthesized and incorporated into the macromolecular network of hydrogels by free radical cross-linking copolymerization. Fluorescence techniques coupled to confocal laser scanning microscopy was employed to characterize the binding and accessibility of the sialyl group in the polymer network by using a monoclonal antibody against GM 3 and the lectin wheat germ agglutinin. The morphology of the network was examined by scanning electron microscopy and confocal microscopy to image the gel morphology. The water content of sialyllactosyl-HPMA hydrogel compared to unmodified gel was characterized by swelling measurements and thermogravimetry. A preliminary implantation study in rat brain was performed to examine the biofunctionality of the sialyllactosyl hydrogel using an experimental model of Parkinson's disease.
本研究描述了具有神经节苷脂GM 3（Neu5Ac-α2,3-Gal-β1,4-Glc）糖基部分本体修饰的聚[N-(2-羟丙基甲基丙烯酰胺)]水凝胶的制备与表征。3'-唾液酸乳糖是一种生物活性表位，可被病毒、细菌和人类细胞（如生长因子受体）上的许多细胞表面受体识别。合成了丙烯酰化的3'-唾液酸乳糖，并通过自由基交联共聚将其掺入水凝胶的大分子网络中。通过共聚焦激光扫描显微镜联用荧光技术，使用抗GM 3单克隆抗体和凝集素麦胚凝集素对聚合物网络中唾液酸基团的结合和可及性进行表征。通过扫描电子显微镜和共聚焦显微镜检查网络形态，以成像凝胶形态。通过溶胀测量和热重分析对唾液酸乳糖基-HPMA水凝胶与未修饰凝胶相比的含水量进行表征。利用帕金森病实验模型进行大鼠脑内初步植入研究，以检验唾液酸乳糖基水凝胶的生物功能。
