Koh T, Maeda T, Nakazawa M, Kani K
Department of Ophthalmology, Shiga University of Medical Science, Ohtsu, Japan.
Nippon Ganka Gakkai Zasshi. 1991 Mar;95(3):249-53.
Immunohistochemical studies of developing rat brains revealed aggregation of serotonin terminal fibers in the visual cortex at from 7 to 14 postnatal days. This aggregation is transient, disappearing 2 weeks after birth. The cells of origin of these serotonin terminals were investigated using double-labeling with retrogradely transported WGA-apoHRP-Au and serotonin immunohistochemistry. WGA-apoHRP-Au was injected into the rat visual cortex on the 9th day after birth. The rats were allowed to survive for 2 days and sacrificed with perfusion. Cryostat sections of the brain were processed with silver-enhancement to develop the retrogradely transported WGA-apoHRP-Au, and then serotonin immunohistochemistry was performed. Double-labeled cells that were labeled with WGA-apoHRP-Au and serotonin-immunoreactive were found mostly in the raphe medianus and raphe dorsalis in rostral and caudal sections of the mesencephalon, respectively.
对发育中的大鼠大脑进行的免疫组织化学研究显示，在出生后7至14天，5-羟色胺终末纤维在视觉皮层聚集。这种聚集是短暂的，出生后2周消失。使用逆行转运的WGA-apoHRP-Au和5-羟色胺免疫组织化学双重标记法研究了这些5-羟色胺终末的起源细胞。在出生后第9天将WGA-apoHRP-Au注入大鼠视觉皮层。让大鼠存活2天，然后灌注处死。大脑的冰冻切片用银增强法处理以显示逆行转运的WGA-apoHRP-Au，然后进行5-羟色胺免疫组织化学。分别在中脑头侧和尾侧切片中，标记有WGA-apoHRP-Au和5-羟色胺免疫反应性的双重标记细胞大多见于中缝正中核和中缝背核。
