Onténiente B, Menétrey D, Arai R, Calas A
Laboratorie Physiologie des Interactions Cellulaires, UA CNRS 339, Talence, France.
Cell Tissue Res. 1989 Jun;256(3):585-92. doi: 10.1007/BF00225608.
The location of the cells giving rise to the methionine-enkephalin (Met-Enk)-ergic innervation of the lateral septal nucleus has been investigated in the rat by combining immunohistochemistry and retrograde axonal tracing. Small volumes (0.06 microliter) of apo-horseradish peroxidase (Apo-HRP) conjugated to wheat-germ agglutinin (WGA) and coupled with colloidal gold particles (WGA-ApoHRP-gold) were injected into the lateral septum. The retrogradely labeled cell bodies were visualized by silver intensification of the gold particles on Vibratome sections that were subsequently processed for immunohistochemistry for Met-Enk. Cells labeled with WGA-ApoHRP-gold were observed in the septal area, throughout the hypothalamus (mainly in the perifornical and lateral nuclei) and in the mesencephalon. The localization of Met-Enk-immunoreactive cells was as previously described. With the exception of a few septal cells close to the injection site, doubly labeled cells were found only in the perifornical nucleus of the hypothalamus. Almost all perifornical magnocellular cells were doubly labeled ipsilateral to the injection site, whereas on the opposite side, only about 25% of the Met-Enk-immunoreactive cells contained WGA-ApoHRP-gold. Other brain regions containing retrogradely labeled or Met-Enk-immunoreactive cells (particularly the raphe nuclei) did not show double-labeled neurons. This study demonstrates, using a new and sensitive technique for specific neurochemical tracing of tracts, that the origin of the Met-Enk-ergic innervation of the rat lateral septal nuclei lies in the magnocellular perifornical nuclei of the hypothalamus. The precise involvement of this pathway in limbic functions remains to be determined.
通过将免疫组织化学与逆行轴突追踪相结合，在大鼠中研究了产生外侧隔核甲硫氨酸脑啡肽（Met-Enk）能神经支配的细胞位置。将与小麦胚芽凝集素（WGA）结合并与胶体金颗粒偶联的小体积（0.06微升）脱辅基辣根过氧化物酶（Apo-HRP）（WGA-ApoHRP-金）注入外侧隔。通过对振动切片机切片上的金颗粒进行银增强来观察逆行标记的细胞体，随后对这些切片进行Met-Enk免疫组织化学处理。在隔区、整个下丘脑（主要在穹窿周核和外侧核）以及中脑观察到用WGA-ApoHRP-金标记的细胞。Met-Enk免疫反应性细胞的定位如先前所述。除了靠近注射部位的少数隔区细胞外，仅在下丘脑穹窿周核中发现了双标记细胞。几乎所有穹窿周大细胞在注射部位同侧都有双标记，而在对侧，只有约25%的Met-Enk免疫反应性细胞含有WGA-ApoHRP-金。其他含有逆行标记或Met-Enk免疫反应性细胞的脑区（特别是中缝核）未显示双标记神经元。本研究使用一种新的、敏感的特定神经化学追踪技术，证明大鼠外侧隔核的Met-Enk能神经支配起源于下丘脑的大细胞穹窿周核。该通路在边缘系统功能中的精确作用仍有待确定。
