Abuladze Tamar, Li Manrong, Menetrez Marc Y, Dean Timothy, Senecal Andre, Sulakvelidze Alexander
Intralytix, Inc, Baltimore, MD 21202, USA.
Appl Environ Microbiol. 2008 Oct;74(20):6230-8. doi: 10.1128/AEM.01465-08. Epub 2008 Aug 22.
A bacteriophage cocktail (designated ECP-100) containing three Myoviridae phages lytic for Escherichia coli O157:H7 was examined for its ability to reduce experimental contamination of hard surfaces (glass coverslips and gypsum boards), tomato, spinach, broccoli, and ground beef by three virulent strains of the bacterium. The hard surfaces and foods contaminated by a mixture of three E. coli O157:H7 strains were treated with ECP-100 (test samples) or sterile phosphate-buffered saline buffer (control samples), and the efficacy of phage treatment was evaluated by comparing the number of viable E. coli organisms recovered from the test and control samples. Treatments (5 min) with the ECP-100 preparation containing three different concentrations of phages (10(10), 10(9), and 10(8) PFU/ml) resulted in statistically significant reductions (P = <0.05) of 99.99%, 98%, and 94%, respectively, in the number of E. coli O157:H7 organisms recovered from the glass coverslips. Similar treatments resulted in reductions of 100%, 95%, and 85%, respectively, in the number of E. coli O157:H7 organisms recovered from the gypsum board surfaces; the reductions caused by the two most concentrated phage preparations were statistically significant. Treatment with the least concentrated preparation that elicited significantly less contamination of the hard surfaces (i.e., 10(9) PFU/ml) also significantly reduced the number of viable E. coli O157:H7 organisms on the four food samples. The observed reductions ranged from 94% (at 120 +/- 4 h posttreatment of tomato samples) to 100% (at 24 +/- 4 h posttreatment of spinach samples). The data suggest that naturally occurring bacteriophages may be useful for reducing contamination of various hard surfaces, fruits, vegetables, and ground beef by E. coli O157:H7.
检测了一种含有三种对大肠杆菌O157:H7具有裂解作用的肌尾噬菌体科噬菌体的噬菌体混合物（命名为ECP - 100），以评估其减少三种该细菌强毒株对硬表面（玻璃盖玻片和石膏板）、番茄、菠菜、西兰花和碎牛肉的实验性污染的能力。用ECP - 100（测试样品）或无菌磷酸盐缓冲盐水缓冲液（对照样品）处理被三种大肠杆菌O157:H7菌株混合物污染的硬表面和食物，并通过比较从测试和对照样品中回收的活大肠杆菌数量来评估噬菌体处理的效果。用含有三种不同浓度噬菌体（10¹⁰、10⁹和10⁸ PFU/ml）的ECP - 100制剂处理（5分钟）后，从玻璃盖玻片中回收的大肠杆菌O157:H7数量分别在统计学上显著减少（P = <0.05）99.99%、98%和94%。类似处理使从石膏板表面回收的大肠杆菌O157:H7数量分别减少100%、95%和85%；两种最高浓度噬菌体制剂引起的减少具有统计学意义。用引起硬表面污染显著较少的最低浓度制剂（即10⁹ PFU/ml）处理也显著减少了四种食物样品上活的大肠杆菌O157:H7数量。观察到的减少范围从94%（番茄样品处理后120±4小时）到100%（菠菜样品处理后24±4小时）。数据表明，天然存在的噬菌体可能有助于减少大肠杆菌O157:H7对各种硬表面、水果、蔬菜和碎牛肉的污染。
