针对小鼠金黄色葡萄球菌的实验性噬菌体疗法。
Experimental phage therapy against Staphylococcus aureus in mice.
作者信息
Capparelli Rosanna, Parlato Marianna, Borriello Giorgia, Salvatore Paola, Iannelli Domenico
机构信息
Dipartimento di Scienze del Suolo, della Pianta, dell'Ambiente e delle Produzioni Animali, Università degli Studi di Napoli Federico II, Via Università 133, Portici, Naples, Italy.
出版信息
Antimicrob Agents Chemother. 2007 Aug;51(8):2765-73. doi: 10.1128/AAC.01513-06. Epub 2007 May 21.
Abstract
The present study describes a bacteriophage (M(Sa)) active against Staphylococcus aureus, including methicillin-resistant staphylococcal strains. When inoculated into mice simultaneously with S. aureus A170 (10(8) CFU/mouse), phage (10(9) PFU) rescued 97% of the mice; when applied to nonlethal (5 x 10(6) CFU/mouse) 10-day infections, the phage also fully cleared the bacteria. The phage M(Sa), delivered inside macrophages by S. aureus, kills the intracellular staphylococci in vivo and in vitro. The phage can also prevent abscess formation and reduce the bacterial load and weight of abscesses. These results suggest a potential use of the phage for the control of both local and systemic human S. aureus infections.
摘要
本研究描述了一种对金黄色葡萄球菌（包括耐甲氧西林葡萄球菌菌株）具有活性的噬菌体（M(Sa)）。当与金黄色葡萄球菌A170（10⁸CFU/小鼠）同时接种到小鼠体内时，噬菌体（10⁹PFU）拯救了97%的小鼠；当应用于非致死性（5×10⁶CFU/小鼠）的10天感染时，噬菌体也完全清除了细菌。由金黄色葡萄球菌递送至巨噬细胞内的噬菌体M(Sa)，在体内和体外均可杀死细胞内的葡萄球菌。该噬菌体还可预防脓肿形成，并减少脓肿的细菌载量和重量。这些结果表明噬菌体在控制人类局部和全身性金黄色葡萄球菌感染方面具有潜在用途。
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