Riches P G, Sheldon J, Smith A M, Hobbs J R
Department of Immunology, Charing Cross and Westminster Medical School, Westminster Hospital, London, UK.
Ann Clin Biochem. 1991 May;28 ( Pt 3):253-9. doi: 10.1177/000456329102800310.
A comparison of paraprotein estimation by scanning densitometry with corresponding immunoglobulin concentration measured by rate immunonephelometry, fixed-time immunonephelometry and kinetic immunoturbidimetry using a large number of sera has indicated overestimation of the immunoglobulin by liquid phase immunoassays in the presence of a monoclonal component. The effect was seen with all three major immunoglobulin isotypes but was most marked with IgM paraproteins when measured by kinetic nephelometry. The overestimations could not be explained by presence of rheumatoid factors or immune complexes in the serum samples, or by non-parallelism, over a range of dilutions, of the reaction of sample and calibrant with the antiserum. The source and species of antiserum used was a major contributory factor.
通过扫描密度测定法估算副蛋白，并与通过速率免疫比浊法、定时免疫比浊法和动态免疫比浊法测量的相应免疫球蛋白浓度进行比较，使用大量血清的研究表明，在存在单克隆成分的情况下，液相免疫测定法会高估免疫球蛋白。在所有三种主要免疫球蛋白同种型中均观察到这种效应，但通过动态比浊法测量IgM副蛋白时最为明显。血清样本中类风湿因子或免疫复合物的存在，或者样本和校准物与抗血清反应在一系列稀释度下的非平行性，均无法解释这种高估现象。所用抗血清的来源和种类是一个主要因素。
