Heinzelmann Larissa, Chagastelles Pedro Cesar, Danilewicz Daniel, Chies José A B, Andrades-Miranda Jaqueline
Programa de Pós-Graduação em Genética e Biologia Molecular, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, 91501-970, Brazil.
J Hered. 2009 Jan-Feb;100(1):119-22. doi: 10.1093/jhered/esn064. Epub 2008 Aug 26.
Despite the recent increase in studies on franciscana dolphin (Pontoporia blainvillei) molecular biology, there has been no published karyotype information, as opportunities for sampling live individuals are very rare. In the present study, the diploid number of the species was established from corneal cell cultures of 2 newborn male franciscanas live stranded (2n = 44). From the comparison of the chromosomal number to the cetacean karyotype phylogeny, we suggest that the most parsimonious hypothesis is that the ancestral character state in the group is the diploid number of 42, with an extra chromosome pair appearing independently twice during cetacean evolution, once in the suborder Odontoceti and once in the suborder Mysticeti. This information on chromosomal number may be useful to future genetic mapping projects of the species.
尽管最近关于弗氏海豚（Pontoporia blainvillei）分子生物学的研究有所增加，但由于获取活体样本的机会非常罕见，目前尚无已发表的核型信息。在本研究中，通过对2头搁浅死亡的新生雄性弗氏海豚的角膜细胞培养，确定了该物种的二倍体数目（2n = 44）。通过将该物种的染色体数目与鲸类核型系统发育进行比较，我们认为最简约的假设是，该类群的祖先特征状态是二倍体数目为42，在鲸类进化过程中，一对额外的染色体独立出现了两次，一次出现在齿鲸亚目，一次出现在须鲸亚目。这些关于染色体数目的信息可能对该物种未来的基因图谱绘制项目有用。
