Laboratório de Mamíferos Aquáticos e Bioindicadores, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil.
Programa de Pós-Graduação em Biodiversidade e Biologia Evolutiva, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Mol Biol Rep. 2021 Mar;48(3):3011-3016. doi: 10.1007/s11033-021-06263-7. Epub 2021 Mar 16.
The franciscana (Pontoporia blainvillei) is the most threatened small cetacean in the South Atlantic. In this study we report the development of 13 microsatellite markers for franciscanas through next-generation sequencing, and the characterization of those loci in 38 samples from the species' northernmost population (Espírito Santo, Brazil). Besides providing diversity indices for the new, specific loci, we also report on the transferability of heterologous loci which had not been screened in franciscanas before, and review all loci used in previous studies. Expected heterozygosity in the new loci ranged between 0.107 and 0.595, and all but one were in Hardy Weinberg Equilibrium. These are the first microsatellite loci isolated from franciscanas, and they are an important addition to heterologous markers that were available previously.
弗朗西斯科鳀（Pontoporia blainvillei）是南大西洋受威胁最大的小型鲸目动物。在这项研究中，我们通过下一代测序开发了 13 个弗朗西斯科鳀微卫星标记，并对来自该物种最北部种群（巴西的圣埃斯皮里图州）的 38 个样本进行了这些基因座的特征描述。除了为新的、特定的基因座提供多样性指数外，我们还报告了之前未在弗朗西斯科鳀中筛选过的异源基因座的可转移性，并回顾了以前研究中使用的所有基因座。新基因座的预期杂合度在 0.107 到 0.595 之间，除一个外，其余均处于 Hardy Weinberg 平衡状态。这些是首次从弗朗西斯科鳀中分离出来的微卫星基因座，它们是之前可用的异源标记的重要补充。
