通过卵子移植获得的F1（BALB/c×NZB）杂交小鼠中自身免疫性溶血性贫血和鼠白血病病毒的传播
Transmission of auto-immune haemolytic anaemia and murine leukaemia virus in F1 (BALB/c X NZB) hybrid mice derived by ovum transplantation.
作者信息
East J, Tuffrey M, Harvey J J, Tilly R J
出版信息
Clin Exp Immunol. 1976 Oct;26(1):148-54.
F1(BALB/c X NZB)hybrid progeny derived by ovum transplantation were used to study the transmission of auto-immune haemolytic anaemia and murine leukaemia virus (MuLV) by male New Zealand black (NZB) mice. Fertilized ova, collected from the normal BALB/c partners 3 1/2 days after mating, were transferred to other, surrogate, BALB/c mothers, which then carried, delivered, and reared the hybrid young. This technical manoeuvre effectively closed the congenital transplacental route theoretically available to any infectious MuLV originating from the NZB father. Nevertheless, such progeny developed exactly the same profile of auto-immune haemolytic disease and the same range of diverse malignancies as their normally-derived F1(BALB/c X NZB) counterparts, and they carried type C MuLV particles readily detectable by electronmicroscopy. We concluded, therefore, that both the auto-immunity and virus were transmitted before placentation, presumably by the NZB male at fertilization, and probably as genetic information.
通过卵子移植获得的F1（BALB/c×NZB）杂交后代被用于研究雄性新西兰黑（NZB）小鼠自身免疫性溶血性贫血和鼠白血病病毒（MuLV）的传播。在交配后3.5天从正常BALB/c配偶体收集的受精卵被转移到其他代孕BALB/c母亲体内，这些母亲随后孕育、分娩并抚养杂交幼崽。这一技术操作有效地阻断了理论上任何源自NZB父亲的感染性MuLV可利用的先天性经胎盘途径。然而，这些后代所患自身免疫性溶血性疾病的特征与正常获得的F1（BALB/c×NZB）对应物完全相同，且患有相同范围的各种恶性肿瘤，并且它们携带通过电子显微镜易于检测到的C型MuLV颗粒。因此，我们得出结论，自身免疫和病毒都是在胎盘形成之前传播的，大概是由NZB雄性在受精时传播的，并且很可能是以遗传信息的形式。
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