Hellsten-Westing Y, Sollevi A, Sjödin B
Department of Physiology III, Karolinska Institute, Stockholm, Sweden.
Eur J Appl Physiol Occup Physiol. 1991;62(5):380-4. doi: 10.1007/BF00634977.
During exhausting exercise adenylate kinase in the muscle cells is activated and a degradation of adenosine 5'-diphosphate occurs. Consequently, degradation products of adenosine 5'-monophosphate including hypoxanthine and uric acid, accumulate in plasma. The aim of this study was to compare the concentration changes of hypoxanthine and uric acid in plasma following running of varying duration and intensity. In addition, plasma creatine kinase activity was measured to assess the possible relationship between metabolic stress and protein release. Four groups of competitive male runners ran 100 m (n = 7), 800 m (n = 11), 5000 m (n = 7) and 42,000 m (n = 7), respectively, at an exhausting pace. Subsequent to the 100 m event (mean running time 11 s) plasma concentrations of hypoxanthine and uric acid increased by 364% and 36% respectively (P less than 0.05), indicating a very high rate of adenine nucleotide degradation during the event. Following the 800-m event (mean running time 125 s), hypoxanthine and uric acid concentrations had increased by 1598% and 66%, respectively (P less than 0.05). Both the events of longer duration, 5000 m and 42,000 m, also caused a significant increase in plasma concentration of hypoxanthine (742% and 237% respectively, P less than 0.05) and plasma uric acid (54% and 34% respectively, P less than 0.05). Plasma activities of creatine kinase were significantly increased at 24 h only following the 5000 m and 42,000 m events (64% and 1186% respectively, P less than 0.05). Changes in plasma creatine kinase activity showed no correlation with changes in plasma concentration of either hypoxanthine or uric acid for the 5000 m and 42,000 m events (r = 0.00-0.45, P greater than 0.05).
在力竭运动期间，肌肉细胞中的腺苷酸激酶被激活，5'-二磷酸腺苷发生降解。因此，包括次黄嘌呤和尿酸在内的5'-单磷酸腺苷降解产物在血浆中积累。本研究的目的是比较不同持续时间和强度跑步后血浆中次黄嘌呤和尿酸的浓度变化。此外，测量血浆肌酸激酶活性以评估代谢应激与蛋白质释放之间的可能关系。四组有竞争力的男性跑步者分别以力竭的速度跑完100米（n = 7）、800米（n = 11）、5000米（n = 7）和42000米（n = 7）。在100米赛事（平均跑步时间11秒）后，血浆中次黄嘌呤和尿酸浓度分别增加了364%和36%（P小于0.05），表明该赛事期间腺嘌呤核苷酸降解率非常高。在800米赛事（平均跑步时间125秒）后，次黄嘌呤和尿酸浓度分别增加了1598%和66%（P小于0.05）。持续时间更长的5000米和42000米赛事也导致血浆次黄嘌呤浓度显著增加（分别为742%和237%，P小于0.05）以及血浆尿酸浓度显著增加（分别为54%和34%，P小于0.05）。仅在5000米和42000米赛事后24小时，血浆肌酸激酶活性显著增加（分别为64%和1186%，P小于0.05）。对于5000米和42000米赛事，血浆肌酸激酶活性的变化与血浆次黄嘌呤或尿酸浓度的变化均无相关性（r = 0.00 - 0.45，P大于0.05）。
