嗜热栖热硫化叶菌EM1中两个与大肠杆菌基因同源的基因的核苷酸序列，这些大肠杆菌基因编码结合蛋白依赖性转运系统的整合膜成分。

Nucleotide sequence of two Clostridium thermosulfurogenes EM1 genes homologous to Escherichia coli genes encoding integral membrane components of binding protein-dependent transport systems.

作者信息

Bahl H, Burchhardt G, Wienecke A

机构信息

Institut für Mikrobiologie, Georg-August-Universität, Göttingen, F.R.G.

出版信息

FEMS Microbiol Lett. 1991 Jun 1;65(1):83-7. doi: 10.1016/0378-1097(91)90476-q.

DOI:10.1016/0378-1097(91)90476-q

Abstract

The complete nucleotide sequence of two genes from Clostridium thermosulfurogenes EM1 homologous to E. coli genes encoding transport proteins was determined by the dideoxy procedure. The genes were cloned from plasmid pCT4, which contains the alpha-amylase gene from C. thermosulfurogenes EM1 as a 2.9-kbp XbaI fragment, inserted into the XbaI site of pUC18, to yield plasmid pCT401. The proteins encoded by the two identified complete ORFs are very hydrophobic and thus are probably integral membrane proteins. They show over 50% similarity to the maltose transport proteins MalF and MalG and to the glycerol-3-phosphate uptake proteins UgpA and UgpE of Escherichia coli. Since these genes are located immediately upstream of the alpha-amylase gene (amyA) of C. thermosulfurogenes EM1, the encoded proteins might be involved in transport of starch degradation products. The genes were tentatively designated amyC and amyD.

摘要

通过双脱氧法测定了嗜热栖热放线菌EM1中两个与大肠杆菌编码转运蛋白的基因同源的基因的完整核苷酸序列。这些基因是从质粒pCT4中克隆出来的，pCT4含有嗜热栖热放线菌EM1的α-淀粉酶基因，作为一个2.9kbp的XbaI片段，插入到pUC18的XbaI位点，得到质粒pCT401。两个已鉴定的完整开放阅读框编码的蛋白质具有很强的疏水性，因此可能是整合膜蛋白。它们与大肠杆菌的麦芽糖转运蛋白MalF和MalG以及甘油-3-磷酸摄取蛋白UgpA和UgpE有超过50%的相似性。由于这些基因位于嗜热栖热放线菌EM1的α-淀粉酶基因（amyA）的紧邻上游，所编码的蛋白质可能参与淀粉降解产物的转运。这些基因被暂定为amyC和amyD。