Dassa E, Hofnung M
EMBO J. 1985 Sep;4(9):2287-93. doi: 10.1002/j.1460-2075.1985.tb03928.x.
The MalG protein is needed for the transport of maltose in Escherichia coli K12. We present the sequence of gene malG. The deduced amino acid sequence corresponds to a protein of 296 amino acid residues (mol. wt. = 32 188 daltons). This protein is largely hydrophobic (hydrophobic index = 0.83) and is thus presumably an integral inner membrane protein which could span the membrane through six hydrophobic segments. We provide direct evidence from fusion proteins for the translation frame and we also identified the in vitro made MalG protein. We have found a sequence which is highly conserved between MalG and MalF, the other integral inner membrane protein of the maltose transport system. This conserved sequence is also present in all known integral membrane proteins of binding protein-dependent transport systems, always at the same distance (approximately 90 residues) from their COOH terminus. We discuss briefly this finding.
MalG蛋白是大肠杆菌K12中麦芽糖转运所必需的。我们给出了malG基因的序列。推导的氨基酸序列对应于一个由296个氨基酸残基组成的蛋白质（分子量 = 32188道尔顿）。该蛋白质在很大程度上是疏水的（疏水指数 = 0.83），因此推测是一种整合内膜蛋白，可能通过六个疏水片段跨越膜。我们从融合蛋白获得了关于翻译框架的直接证据，并且还鉴定了体外制备的MalG蛋白。我们发现了一个在MalG和MalF（麦芽糖转运系统的另一种整合内膜蛋白）之间高度保守的序列。这个保守序列也存在于所有已知的依赖结合蛋白的转运系统的整合膜蛋白中，总是与其COOH末端保持相同的距离（约90个残基）。我们简要讨论了这一发现。
