Ayadi Wajdi, Karray-Hakim Héla, Khabir Abdelmajid, Feki Lamia, Charfi Slim, Boudawara Tahia, Ghorbel Abdelmonem, Daoud Jamel, Frikha Mounir, Busson Pierre, Hammami Adnane
Laboratoire de Microbiologie, Hôpital Habib Bour-guiba, 3029 Sfax, Tunisie.
Anticancer Res. 2008 Jul-Aug;28(4B):2161-7.
Aberrant methylation of tumor suppressor gene (TSG) promoters has been extensively investigated in nasopharyngeal carcinomas (NPC) from South East Asia but not from North Africa.
The methylation status of p16, deleted in lung and esophageal cancer (DLEC1), zinc finger, MYND-type containing 10 (BLU) and E-cadherin gene promoters was investigated in 44 Tunisian NPC biopsies and three NPC xenografts, by methylation-specific PCR (MSP) combined with a quantitative assessment of some of the samples.
The frequencies of aberrant promoter methylation were similar to previous figures reported for Asian series: p16 27/44 (65%), DLEC1 38/44 (86.3%), BLU 15/44 (34.1%) and E-cadherin 35/44 (79.5%). Although in other malignancies, aberrant promoter hypermethylation increases with patient age, it was at the same high frequency in the juvenile and adult forms of Tunisian NPCs. However, there was a strong association between aberrant methylation of E-cadherin promoter and lymph node invasion (p < 0.01). In addition, aberrant methylation of the BLU promoter was significantly correlated with an undifferentiated histological type (p = 0.03).
Aberrant methylation of tumor suppressor genes occurs with the same high frequency in NPCs from North Africa as in South East Asia, regardless of patient age.
抑癌基因（TSG）启动子的异常甲基化在东南亚鼻咽癌（NPC）中已得到广泛研究，但在北非尚未开展。
采用甲基化特异性PCR（MSP）结合部分样本的定量评估，对44例突尼斯NPC活检组织和3例NPC异种移植物中p16、肺癌和食管癌缺失基因（DLEC1）、含锌指结构和MYND结构域蛋白10（BLU）以及E-钙黏蛋白基因启动子的甲基化状态进行研究。
启动子异常甲基化频率与先前报道的亚洲系列相似：p16为27/44（65%），DLEC1为38/44（86.3%），BLU为15/44（34.1%），E-钙黏蛋白为35/44（79.5%）。尽管在其他恶性肿瘤中，启动子异常高甲基化随患者年龄增加，但在突尼斯NPC的青少年和成人形式中，其频率相同。然而，E-钙黏蛋白启动子异常甲基化与淋巴结转移之间存在强关联（p < 0.01）。此外，BLU启动子异常甲基化与未分化组织学类型显著相关（p = 0.03）。
无论患者年龄如何，北非NPC中抑癌基因的异常甲基化频率与东南亚相同。
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