Lao T D, Truong P K, Thieu H H, Nguyen D H, Nguyen M T, Le Tah
Department of Pharmaceutical and Medical Biology, Ho Chi Minh City Open University, Ho Chi Minh City, Vietnam.
Ear, Nose and Throat Department, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam.
Balkan J Med Genet. 2021 Jul 27;24(1):57-66. doi: 10.2478/bjmg-2021-0005. eCollection 2021 Jun.
The phenome of gene methylation and the expression of latent membrane protein 1 () gene are associated with nasopharyngeal carcinoma (NPC). In order to determine whether cooperative expression or methylation of could serve as the potential molecule biomarker target for diagnosis and therapy of NPC, a case-control study including 93 NPC biopsy samples and 100 non cancerous nasopharyngeal swab samples were examined, as well as the strength of association among them by the quantitative polymerase chain reaction (qPCR) and nested-methylation-specific PCR methods. The significantly higher frequency of expression and methylation in NPC biopsy samples, accounting for 76.34 and 73.12%, respectively, compared to non cancerous samples, accounting for 0.00 and 30.00%, respectively, were observed. The significant correlation between the expression and methylation in NPC samples was reported. In detail, in the stage IV of NPC, in case of -positive samples, 35 of 37 samples (accounting for 94.60%) were positive for methylation of . It was demonstrated that cooperative expression and gene methylation could serve as a molecular biomarker in NPC.
基因甲基化表型与潜伏膜蛋白1（LMP1）基因的表达与鼻咽癌（NPC）相关。为了确定LMP1的协同表达或甲基化是否可作为NPC诊断和治疗的潜在分子生物标志物靶点，我们进行了一项病例对照研究，检测了93份NPC活检样本和100份非癌性鼻咽拭子样本，并通过定量聚合酶链反应（qPCR）和巢式甲基化特异性PCR方法检测了它们之间的关联强度。结果观察到，NPC活检样本中LMP1表达和甲基化的频率显著更高，分别为76.34%和73.12%，而非癌性样本中分别为0.00%和30.00%。研究报道了NPC样本中LMP1表达与甲基化之间存在显著相关性。具体而言，在NPC的IV期，在LMP1阳性样本中，37份样本中有35份（占94.60%）LMP1甲基化呈阳性。结果表明，LMP1的协同表达和基因甲基化可作为NPC的分子生物标志物。
