Barbanis Sotirios, Ioannou Maria, Kouvaras Evangelos, Karasavvidou Foteini, Nakou Marianna, Papamichali Roidoula, Koukoulis George
Department of Pathology, Medical School, University of Thessaly, 411 10, Larissa, Thessaly, Greece.
Pathol Oncol Res. 2009 Mar;15(1):11-7. doi: 10.1007/s12253-008-9094-0. Epub 2008 Aug 28.
Inner centromere protein (INCENP) is a member of the Chromosomal Passenger Complex (CPC), which is a four member protein complex essential for proper completion of mitosis and cell division (cytokinesis). Inappropriate chromosomal segregation and cytokinesis due to deregulated expression of chromosome passenger proteins may lead to aneuploidy and cancer including lymphomas. According to our knowledge this is the first study investigating immunohistochemical expression of INCENP in lymphoma cases and cancer tissues in general. Our purpose was to characterize the expression of INCENP in cases of non-Hodgkin B-cell lymphomas, to compare the immunoreactivity between low and high grades and to evaluate the correlation between INCENP and MIB-1 labeling indices. We examined INCENP and MIB-1 immunoreactivity in paraffin sections of 55 samples of non-Hodgkin B-cell lymphomas, obtained from 55 patients, 31 men and 24 women. Thirty were of high grade and 25 were of low grade. Our results showed significantly higher nuclear immunohistochemical expression of INCENP in high grade B-cell lymphomas versus low grade ones. Also INCENP expression was significantly correlated with MIB-1 labeling index. Taken together our results point to a possible association between increased INCENP immunostaining and B-cell lymphoma aggressiveness and also stress the need for further investigating the expression of INCENP and other mitotic regulatory proteins in lymphomas and other malignant neoplasms.
着丝粒内蛋白（INCENP）是染色体乘客复合体（CPC）的成员之一，该复合体是一种由四个成员组成的蛋白质复合体，对于有丝分裂和细胞分裂（胞质分裂）的正常完成至关重要。由于染色体乘客蛋白的表达失调而导致的不适当染色体分离和胞质分裂可能会导致非整倍体和包括淋巴瘤在内的癌症。据我们所知，这是第一项研究INCENP在淋巴瘤病例及一般癌症组织中的免疫组化表达的研究。我们的目的是描述INCENP在非霍奇金B细胞淋巴瘤病例中的表达特征，比较低级别和高级别之间的免疫反应性，并评估INCENP与MIB-1标记指数之间的相关性。我们检测了从55例患者（31名男性和24名女性）获取的55份非霍奇金B细胞淋巴瘤石蜡切片中INCENP和MIB-1的免疫反应性。其中30例为高级别，25例为低级别。我们的结果显示，与低级别B细胞淋巴瘤相比，高级别B细胞淋巴瘤中INCENP的核免疫组化表达显著更高。此外，INCENP的表达与MIB-1标记指数显著相关。综上所述，我们的结果表明INCENP免疫染色增加与B细胞淋巴瘤侵袭性之间可能存在关联，同时也强调了进一步研究INCENP和其他有丝分裂调节蛋白在淋巴瘤及其他恶性肿瘤中表达的必要性。
