Csete Béla, Lengyel Zsuzsanna, Kádár Zsolt, Battyáni Zita
Department of Dermatology, University of Pécs, 20. Kodály Z. u., Pécs, Hungary.
Pathol Oncol Res. 2009 Mar;15(1):47-53. doi: 10.1007/s12253-008-9086-0. Epub 2008 Aug 28.
Poly(adenosine diphosphate-ribose) polymerases (PARPs) are a family of enzymes, which catalyses poly (ADP-ribosyl)ation of DNA-binding proteins and directly involved in genomic stability, DNA repair, and apoptosis. In this study, we evaluated the immunomorphology of PARP-1 in melanoma and its prognostic importance. We studied PARP-1 expression by immunohistochemistry in a selected series of 54 primary cutaneous malignant melanoma (CMM). The findings of the present study suggest that the neoplastic progression toward the invasive (both horizontal and vertical) growth phase of CMM cells is characterized by the loss of cleavage of PARP-1, probably signaling an imbalance of the apoptotic process in these cells and leading to further gain to aggression. Over-expression of full-length PARP-1 was correlated with recurrence and/or progression of the disease and so act as a promising new biological marker of CMM. Our study represents the evidence of a direct correlation between the PARP-1-mediated apoptotic process and the biologic behavior of CMM.
聚（腺苷二磷酸核糖）聚合酶（PARP）是一类酶，可催化DNA结合蛋白的聚（ADP-核糖基）化，并直接参与基因组稳定性、DNA修复和细胞凋亡。在本研究中，我们评估了PARP-1在黑色素瘤中的免疫形态学及其预后意义。我们通过免疫组织化学研究了54例原发性皮肤恶性黑色素瘤（CMM）的选定系列中PARP-1的表达。本研究结果表明，CMM细胞向侵袭性（水平和垂直）生长阶段的肿瘤进展的特征是PARP-1裂解的丧失，这可能表明这些细胞中凋亡过程的失衡，并导致进一步的侵袭性增加。全长PARP-1的过度表达与疾病的复发和/或进展相关，因此可作为CMM有前景的新生物标志物。我们的研究代表了PARP-1介导的凋亡过程与CMM生物学行为之间直接相关性的证据。
