Gimenes F, Takeda K I, Fiorini A, Gouveia F S, Fernandez M A
Departamento de Biologia Celular e Genética, Universidade Estadual de Maringá, Maringá, PR, Brasil.
Genet Mol Res. 2008 Jun 24;7(2):549-58. doi: 10.4238/vol7-2gmr461.
Intrinsically bent DNA is an alternative conformation of the DNA molecule caused by the presence of dA/dT tracts, 2 to 6 bp long, in a helical turn phase DNA or with multiple intervals of 10 to 11 bp. Other than flexibility, intrinsic bending sites induce DNA curvature in particular chromosome regions such as replication origins and promoters. Intrinsically bent DNA sites are important in initiating DNA replication, and are sometimes found near to regions associated with the nuclear matrix. Many methods have been developed to localize bent sites, for example, circular permutation, computational analysis, and atomic force microscopy. This review discusses intrinsically bent DNA sites associated with replication origins and gene promoter regions in prokaryote and eukaryote cells. We also describe methods for identifying bent DNA sites for circular permutation and computational analysis.
固有弯曲DNA是DNA分子的一种替代构象，由螺旋转角相DNA中存在的2至6个碱基对长的dA/dT序列或具有多个10至11个碱基对的间隔引起。除了灵活性之外，固有弯曲位点还会在特定的染色体区域（如复制起点和启动子）诱导DNA弯曲。固有弯曲DNA位点在启动DNA复制中很重要，有时会在与核基质相关的区域附近发现。已经开发了许多方法来定位弯曲位点，例如，环形置换、计算分析和原子力显微镜。本综述讨论了原核生物和真核生物细胞中与复制起点和基因启动子区域相关的固有弯曲DNA位点。我们还描述了用于通过环形置换和计算分析识别弯曲DNA位点的方法。
