Miranda C, Vieira M J, Silva E, Carvalheira J, Parrish C R, Thompson G
Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto, Porto, Portugal.
Rede de Investigação em Biodiversidade e Biologia Evolutiva (CIBIO/InBIO), Laboratório Associado, Universidade do Porto, Vairão, Portugal.
Transbound Emerg Dis. 2017 Aug;64(4):1178-1183. doi: 10.1111/tbed.12483. Epub 2016 Feb 29.
Feline panleukopenia virus (FPV) and canine parvovirus (CPV) are two closely related viruses, which cause acute gastroenteritis in carnivores, and cats may be infected by strains of both viruses. The viruses are found worldwide and may have changing host ranges and genetic variation that can be found around the world in some cases. Here, we screened a Portuguese population of cats by a conventional PCR assay for the presence of FPV/CPV viruses in faecal samples and tissues between 2006-2008 and 2012-2014. The sequence analysis of the complete VP2 gene showed that 18 of 31 animals tested were positive for FPV DNA, and no case of CPV infection was detected. The analysis of specific DNA detected three new non-synonymous substitutions in the VP2 gene that were found in single groups and were related to viruses reported elsewhere by phylogenetic analysis - some were related to Italian isolates, one was closely related to isolates from Vietnam and China, and two were related with older strains from the USA. The results of our study show that FPV circulated in the Portuguese cat population and as expected the found strains are slightly divergent from those reported previously.
猫泛白细胞减少症病毒（FPV）和犬细小病毒（CPV）是两种密切相关的病毒，它们可导致食肉动物患急性肠胃炎，猫可能会感染这两种病毒的毒株。这两种病毒在全球范围内均有发现，在某些情况下，其宿主范围可能会发生变化，并且在世界各地都可能存在基因变异。在此，我们在2006 - 2008年以及2012 - 2014年期间，通过常规PCR检测法对葡萄牙的猫群进行了筛查，以检测粪便样本和组织中是否存在FPV/CPV病毒。对完整VP2基因的序列分析表明，在检测的31只动物中，有18只的FPV DNA呈阳性，未检测到CPV感染病例。对特定DNA的分析在VP2基因中检测到三个新的非同义替换，这些替换出现在单个组中，并且通过系统发育分析发现与其他地方报道的病毒有关——有些与意大利分离株有关，一个与来自越南和中国的分离株密切相关，还有两个与美国的较旧毒株有关。我们的研究结果表明，FPV在葡萄牙猫群中传播，并且正如预期的那样，所发现的毒株与先前报道的毒株略有不同。
