Xue Haowen, Liang Yang, Gao Xu, Song Yanhao, Zhu Kunru, Yang Meng, Hao Jingrui, Ma Haoyuan, Yu Kai
Laboratory for Animal Molecular Virology, Department of Veterinary Medicine, College of Agricultural, Yanbian University, Yanji 133002, China.
Beijing Shengzetang Animal Hospital, Beijing 102218, China.
Vet Sci. 2023 Jul 6;10(7):440. doi: 10.3390/vetsci10070440.
Feline panleukopenia (FP) is a severe viral illness caused by the feline panleukopenia virus (FPV), putting sectors like companion cat breeding and endangered feline conservation at risk. The virus has a high morbidity and fatality rate and is found all over the world. We created a novel FPV assay using nanoPCR technology and assessed the method's specificity and sensitivity. The approach amplified a 345 bp nucleic acid fragment with a minimum detection limit of 7.97 × 10 copies/μL, which is about 100 times greater than traditional PCR. We collected anal swabs from 83 cats suspected of FPV infection for practical application, and the FPV-positive rate determined by the nanoPCR approach was 77.1%. In conclusion, the approach is more sensitive than conventional PCR and more convenient and cost-effective than qPCR methodology and may be utilized for the clinical detection of FPV.
猫泛白细胞减少症（FP）是由猫泛白细胞减少症病毒（FPV）引起的一种严重病毒性疾病，使伴侣猫繁育和濒危猫科动物保护等领域面临风险。该病毒具有高发病率和死亡率，在世界各地均有发现。我们利用纳米PCR技术创建了一种新型FPV检测方法，并评估了该方法的特异性和灵敏度。该方法扩增出一个345 bp的核酸片段，最低检测限为7.97×10拷贝/μL，约为传统PCR的100倍。我们收集了83只疑似感染FPV的猫的肛门拭子用于实际应用，通过纳米PCR方法确定的FPV阳性率为77.1%。总之，该方法比传统PCR更灵敏，比qPCR方法更方便且成本效益更高，可用于FPV的临床检测。
