Xiao Bo, Li Qingwang, Feng Bo, Han Zengsheng, Gao Dawei, Zhao Rui, Li Jian, Li Kun, Zhi Xubo, Yang Hai, Liu Zhibin
College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China.
Appl Biochem Biotechnol. 2009 Jun;157(3):357-66. doi: 10.1007/s12010-008-8346-5. Epub 2008 Aug 27.
For producing a large quantity of human nerve growth factor beta (hNGF-beta), the recombinant adenovirus containing an expression cassette of hNGF-beta complementary DNA gene was constructed and then instilled directly into goats' mammary glands. The recombinant hNGF-beta was detected in the milk. The maximal expression level of recombinant hNGF-beta in the milk reached 196.8 mg/l. The biological activity of the recombinant hNGF-beta, compared with the commercial hNGF-beta, was tested using PC12 cells. The results demonstrate that instilling recombinant adenovirus directly into the mammary gland of goat is an efficient approach to producing a large quantity of hNGF-beta.
为了大量生产人神经生长因子β（hNGF-β），构建了含有hNGF-β互补DNA基因表达盒的重组腺病毒，然后将其直接注入山羊乳腺。在乳汁中检测到了重组hNGF-β。乳汁中重组hNGF-β的最大表达水平达到196.8毫克/升。使用PC12细胞测试了重组hNGF-β与市售hNGF-β相比的生物活性。结果表明，将重组腺病毒直接注入山羊乳腺是大量生产hNGF-β的有效方法。
