Han Zeng-Sheng, Li Qing-Wang, Zhang Zhi-Ying, Xiao Bo, Gao Da-Wei, Wu Shu-Yun, Li Jian, Zhao Hong-Wei, Jiang Zhong-Liang, Hu Jian-Hong
College of Animal Science and Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province 712100, PR China.
Protein Expr Purif. 2007 May;53(1):225-31. doi: 10.1016/j.pep.2006.11.019. Epub 2006 Dec 8.
The expression of human lactoferrin in the mammary gland is an attractive approach to diminish its current production cost. Previous attempts to produce lactorferrin in the milk of transgenic animals resulted in very high cost and uncertain results. In this paper, we have directly infused replication-defective adenovirus encoding human lactoferrin cDNA into the mammary gland of goats via the teat canal. In this way, we obtained a high level of expressed human lactoferrin up to 2g/L in the milk of goats. The milk serum was collected from the infected mammary gland 48 h post-infection and subjected to a 10% SDS-PAGE and Western blotting. A approximately 80-kDa protein was visualized after viral vector infection. Our results demonstrate that intraductal injection of recombinant replication-defective adenovirus vectors may provide a very useful tool for large-scale production of recombinant proteins of biopharmaceutical interest.
人乳铁蛋白在乳腺中的表达是降低其当前生产成本的一种有吸引力的方法。以前在转基因动物乳汁中生产乳铁蛋白的尝试导致成本非常高且结果不确定。在本文中，我们通过乳头管将编码人乳铁蛋白cDNA的复制缺陷型腺病毒直接注入山羊乳腺。通过这种方式，我们在山羊乳汁中获得了高达2g/L的高水平表达的人乳铁蛋白。在感染后48小时从受感染的乳腺收集乳汁血清，并进行10% SDS-PAGE和蛋白质印迹分析。病毒载体感染后可见一条约80 kDa的蛋白质条带。我们的结果表明，导管内注射重组复制缺陷型腺病毒载体可能为大规模生产具有生物制药意义的重组蛋白提供一个非常有用的工具。
