Chidley Christopher, Mosiewicz Katarzyna, Johnsson Kai
Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering, CH-1015 Lausanne, Switzerland.
Bioconjug Chem. 2008 Sep;19(9):1753-6. doi: 10.1021/bc800268j. Epub 2008 Aug 28.
Bioconjugations often rely on adaptor molecules to cross-link different biomolecules. In this work, we introduce the molecular adaptor covalin, which is a protein chimera of two self-labeling proteins with nonoverlapping substrate specificity. Covalin permits a selective and covalent heteroconjugation of biomolecules displaying appropriate functional groups. Examples for the use of covalin include the specific heteroconjugation of a reporter enzyme to an antibody and of molecular probes to the surface of living cells. The efficiency and specificity of covalin-based bioconjugations together with the availability of a large variety of substrates create immediate and ubiquitous applications for covalin in bioconjugate chemistry.
生物共轭通常依赖于衔接分子来交联不同的生物分子。在这项工作中，我们引入了分子衔接物共价蛋白，它是两种具有不重叠底物特异性的自标记蛋白的蛋白质嵌合体。共价蛋白允许对显示适当官能团的生物分子进行选择性和共价异源共轭。共价蛋白的应用实例包括将报告酶与抗体特异性异源共轭，以及将分子探针与活细胞表面异源共轭。基于共价蛋白的生物共轭的效率和特异性，以及大量可用底物的存在，使得共价蛋白在生物共轭化学中立即得到广泛应用。
