Chemistry Department, University of California, Davis, CA 95616, USA.
Curr Opin Chem Biol. 2010 Dec;14(6):803-9. doi: 10.1016/j.cbpa.2010.09.015. Epub 2010 Oct 15.
Notable new applications of antibodies for imaging involve genetically extracting the essential molecular recognition properties of an antibody, and in some cases enhancing them by mutation, before protein expression. The classic paradigm of intravenous administration of a labeled antibody to image not only its target but also its metabolism can be improved on. Protocols involving molecular targeting with an engineered unlabeled protein derived from an antibody, followed by capture of a small probe molecule that provides a signal, are being developed to a high level of utility. This is accompanied by new strategies for probe capture such as irreversible binding, incorporation of engineered enzyme active sites, and antibody-ligand systems that generate a signal only upon binding or uptake.
在蛋白质表达之前，通过基因提取抗体的基本分子识别特性，并在某些情况下通过突变来增强这些特性。静脉注射标记抗体以不仅对其靶标，而且对其代谢物进行成像的经典范例可以得到改进。涉及使用源自抗体的工程化未标记蛋白进行分子靶向，然后捕获提供信号的小探针分子的方案正在被开发并具有很高的实用性。同时，还出现了一些新的探针捕获策略，例如不可逆结合、工程酶活性位点的掺入以及仅在结合或摄取时才产生信号的抗体配体系统。
